HBeAg阴性慢性乙型肝炎患者B、C基因型 HBV S蛋白变异比较

肝脏 ›› 2021, Vol. 26 ›› Issue (8): 847-850.

HBeAg阴性慢性乙型肝炎患者B、C基因型 HBV S蛋白变异比较

章晓鹰, 顾超, 马道亮, 张珏, 高月求, 孙学华

201203 上海中医药大学附属曙光医院检验科(章晓鹰,顾超,张珏),肝病科(高月求,孙学华);广州立菲达安技术有限公司(马道亮)

收稿日期:2020-09-29 出版日期:2021-08-31 发布日期:2021-09-29
通讯作者: 孙学华,Email:13585769286@163.com
基金资助:
上海申康市级医院项目(SHDC12016121)

Comparison of mutation rate of HBV S protein between genotype B and C in HBeAg negative chronic hepatitis B patients

ZHANG Xiao-ying¹, GU Chao¹, MA Dao-liang³, ZHANG Jue¹, Gao Yue-qiu², SUN Xue-hua²

1. Department of Clinical Laboratory, Shanghai University of Traditional Chinese Medical attached Shu-guang Hospital, Shanghai 201203, China;
2. Department of Hepotology, Shanghai University of Traditional Chinese Medical attached Shu-guang Hospital, Shanghai 201203, China

Received:2020-09-29 Online:2021-08-31 Published:2021-09-29
Contact: SUN Xue-hua, Email: 13585769286@163.com

摘要/Abstract

摘要： 目的探讨乙型肝炎病毒(hepatitis B virus,HBV)基因型与S蛋白变异的关联,为分析病毒基因型在疾病预后转归中的作用提供理论基础。方法选择上海中医药大学附属曙光医院东院2019年1月至2019年10月门诊或住院的HBeAg阴性慢性乙型肝炎患者99例,其中,B基因型50例,C基因型49例。基因测序法用于S蛋白基因序列分析,分类变量间差异分析采用χ²检验与连续校准。非正态分布数据采用非参数秩和检验。结果 HBeAg阴性慢性乙型肝炎组C基因型的S蛋白变异率高于B基因型为87.76%(43/49)比66.00%(33/50),(χ² =6.567,P=0.010),且变异差异位点似乎为随机分布,主要亲水区 (major hydrophilic region,MHR )与MHR外的变异率,C基因型均高于B基因型为38.78%(19/49)比18.00%(9/50),(χ² =5.266, P=0.022);85.71%(42/49)比56.00%(28/50),(χ² =10.550,P=0.001)。两基因型MHR的“a”决定簇[32.65%(16/49)比12.00%(6/50),(χ²=6.107,P=0.013)]以及MHR外的细胞毒T淋巴细胞+辅助性T淋巴细胞(CTL+Th)免疫表位[69.39%(34/49)比40.00%(20/50),(χ²=8.620,P=0.003)]与非免疫表位[53.06%(24/49)比30.00%(15/50),(χ²=5.424,P=0.020)]差异均有统计学意义。结论 HBeAg阴性慢性乙型肝炎患者C基因型较B基因型更容易产生HBV S蛋白变异。

关键词: 乙型肝炎病毒, S蛋白, 乙型肝炎病毒基因型, HBeAg阴性慢性乙型肝炎

Abstract: Objective To investigate the association between hepatitis B virus (HBV) genotype and S protein mutations and to provide a theoretical basis for analyzing the role of viral genotype in the prognosis of disease.Methods Ninty-nine HBeAg negative chronic hepatitis B (CHB) patients admitted to our hospital from January 2019 to October 2019 were enrolled in this study, the number of genotype B and genotype C were 50 and 49, respectively. DNA sequencing was used to detect the gene sequence of HBV S protein. Differences between categorical variables were analyzed by Chi-square test and Continuity Correction. Non-parametric test (Mann-Whitney U) was used for analyzing non-normal distribution data.Results The mutation rate of S protein in genotype C group was significantly higher that of the genotype B group (87.76% vs.66.00% , χ²=6.567, P=0.010), furthermore, the mutation sites were distributed randomly, mutation rates in both major hydrophilic region (MHR) and outside the MHR of genotype C were higher than that of genotype B (38.78% vs.18.00%, χ²=5.266, P=0.022; 85.71% vs.56.00%, χ²=10.550, P=0.001 respectively). In addition, statistical differences were also found in the “a”determinant (32.65% vs.12.00% , χ²=6.107, P=0.013) located in MHR and immune epitopes (69.39% vs.40.00% , χ²=8.620, P=0.003) and nonimmune epitopes (53.06% vs.30.00%, χ²=5.424, P=0.020) of cytotoxic T lymphocytes + T-helper cell (CTL + Th ) located outside the MHR between the genotype C and B.Conclusion Genotype C was more prone to HBV S protein mutations than genotype B among HBeAg negative CHB patients.

Key words: Hepatitis B virus, S protein, Hepatitis B virus genotype, HBeAg negative chronic hepatitis B

章晓鹰, 顾超, 马道亮, 张珏, 高月求, 孙学华. HBeAg阴性慢性乙型肝炎患者B、C基因型 HBV S蛋白变异比较[J]. 肝脏, 2021, 26(8): 847-850.

ZHANG Xiao-ying, GU Chao, MA Dao-liang, ZHANG Jue, Gao Yue-qiu, SUN Xue-hua. Comparison of mutation rate of HBV S protein between genotype B and C in HBeAg negative chronic hepatitis B patients[J]. Chinese Hepatolgy, 2021, 26(8): 847-850.

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