miR-548a-5p靶向HMBOX1调控肝癌细胞HepG2的凋亡研究

肝脏 ›› 2021, Vol. 26 ›› Issue (8): 871-873.

miR-548a-5p靶向HMBOX1调控肝癌细胞HepG2的凋亡研究

袁溢苒, 陈龙, 张臻

637000 四川南充市中心医院特需医疗科(袁溢苒),消化内科(陈龙);南充市卫生健康委员会(张臻)

收稿日期:2020-11-22 出版日期:2021-08-31 发布日期:2021-09-29

miR-548a-5p regulates the apoptosis of hepatocellular carcinoma cell line HepG2 by targeting HMBOX1

YUAN Yi-ran¹, CHEN Long², ZHANG Zhen³

1. Nanchong Central Hospital Special Medical Department, Nanchong, Sichuan 637000, China;
2. Department of Digestive Medicine, Nanchong Central Hospital;
3. Nanchong Health Committee Nanchong, Sichuan 637000, China

Received:2020-11-22 Online:2021-08-31 Published:2021-09-29

摘要/Abstract

摘要： 目的探讨miR-548a-5p调控肝癌细胞HepG2凋亡的潜在机制。方法通过miR-548a-5p mimics和miR-548a-5p inhibitor分别过表达和敲低miR-548a-5p,检测HepG2的凋亡水平。通过miRDB在线分析和荧光素酶报告系统检测miR-548a-5p的潜在底物。通过siRNA和PCDNA3.1载体过表达底物,检测HepG2的凋亡水平。结果过表达miR-548a-5p后,HepG2的凋亡水平下降,miR-548a-5pd的潜在靶向HMBOX1的表达量显著降低;相反,在敲低miR-548a-5p后,HepG2的凋亡水平上升,HMBOX1的表达量显著上升(P<0.05)。荧光素酶报告系统显示miR-548a-5p靶向HMBOX1的3端非编码区,在敲低HMBOX1后,HepG2的凋亡水平明显下降,过表达后则相反(P<0.05)。结论 miR-548a-5p通过靶向HMBOX1的mRNA的3端非编码区抑制HMBOX1的翻译,从而抑制肝癌细胞HepG2的凋亡。

关键词: miR-548a-5p, HMBOX1, 肝癌, HepG2, 凋亡

Abstract: Objective To investigate the potential mechanism of mir-548a-5p regulating the apoptosis of hepatocellular carcinoma (HCC) cell line HepG2.Methods MiR-548a-5p mimics and miR-548a-5p inhibitor were used to overexpress and knockdown miR-548a-5p and the apoptosis levels of HepG2 were detected. Potential substrates of miR-548a-5p were detected by miRDB online analysis and luciferase reporting system. The siRNA and PCDNA3.1 vector were used to overexpress substrates and the apoptosis level of HepG2 was detected.Results After overexpressing miR-548a-5p, the apoptosis level of HepG2 decreased, and the expression level of HMBOX1, a potential target of miR-548a-5p, decreased significantly. On the contrary, after knocking down miR-548a-5p, the apoptosis level of HepG2 increased, and the expression of HMBOX1 increased significantly (P<0.05). Luciferase reporter system showed that miR-548a-5p targeted the 3-terminal non-coding region of HMBOX1. After knocking down HMBOX1, the apoptosis level of HepG2 decreased significantly, and vice versa (P<0.05).Conclusion MiR-548a-5p inhibits the translation of HMBOX1 by targeting the 3-terminal non-coding region of HMBOX1 mRNA, thereby inhibiting the apoptosis of HepG2 of HCC cells.

Key words: MiR-548a-5p, HMBOX1, Hepatocellular carcinoma, HepG2, Apoptosis

袁溢苒, 陈龙, 张臻. miR-548a-5p靶向HMBOX1调控肝癌细胞HepG2的凋亡研究[J]. 肝脏, 2021, 26(8): 871-873.

YUAN Yi-ran, CHEN Long, ZHANG Zhen. miR-548a-5p regulates the apoptosis of hepatocellular carcinoma cell line HepG2 by targeting HMBOX1[J]. Chinese Hepatolgy, 2021, 26(8): 871-873.

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