慢性乙型肝炎患者血清HBV pgRNA与HBV DNA和HBeAg水平变化关系研究

摘要/Abstract

摘要： 目的研究慢性乙型肝炎(CHB)患者血清乙型肝炎病毒前基因组RNA(HBV pgRNA)与乙型肝炎病毒基因(HBV DNA)和乙型肝炎e抗原(HBeAg)水平变化关系。方法选择2018年6月至2021年6月收治的72例CHB患者作为研究对象,以样本值/临界值(S/CO)作为HBeAg检测的结果,将1＜S/CO≤5的患者纳入低浓度组(16例),5＜S/CO≤10的患者纳入中浓度组(35例),＞10的患者纳入高浓度组(21例)。定量检测三组HBV pgRNA、HBV DNA、HBeAg水平,分析HBV pgRNA、HBV DNA水平与HBeAg水平的相关性。结果三组HBV pgRNA水平分别为(5.85±0.64)拷贝/mL、(7.12±0.38)拷贝/mL、(7.75±0.59)拷贝/mL,三组HBV DNA水平分别为(5.34±0.49)拷贝/mL、(7.16±0.62)拷贝/mL、(9.35±0.54)拷贝/mL,三组HBV pgRNA、HBV DNA水平比较,均有低浓度组＜中浓度组＜高浓度组,P<0.05;经相关性分析,HBV pgRNA与HBeAg呈正相关(r=0.566,P=0.002),HBV DNA和HBeAg呈正相关(r=0.496,P=0.005)。结论随着HBeAg水平的增加,HBV pgRNA、HBV DNA水平逐渐提升,CHB患者血清HBV pgRNA、HBV DNA水平和HBeAg水平呈正相关,临床可结合三项指标对HBV的感染和复制、CHB患者的病情进行更加准确地诊断。

关键词: 慢性乙型肝炎, 乙型肝炎病毒前基因组RNA, 乙肝病毒基因, 乙型肝炎e抗原

Abstract: Objective To study on the relationship between the levels of pregenomic RNA of hepatitis B virus (HBV pgRNA), DNA of hepatitis B virus (HBV DNA) and e antigen of hepatitis B (HBeAg) in patients with chronic hepatitis B (CHB).Methods A total of 72 CHB patients admitted from June 2018 to June 2021 were selected as the studied subjects, and the sample value/critical value (S/CO) was used as the result of HBeAg. Patients with 1<S/CO≤5 were included in the low-concentration group (n=16 cases). Patients with 5<S/CO≤10 were included in the medium-concentration group (n=35 cases). Patients with S/CO>10 were included in the high-concentration group (n=21 cases). The levels of HBV pgRNA, HBV DNA and HBeAg in three groups of patients were quantitatively detected, and the correlations between HBV pgRNA, HBV DNA and HBeAg was analyzed.Results The levels of HBV pgRNA in three groups of patients were (5.85±0.64) copies/mL, (7.12±0.38) copies/mL, (7.75±0.59) copies/mL, respectively. The HBV DNA levels were (5.34±0.49) copies/mL, (7.16±0.62) copies/mL, (9.35±0.54) copies/mL, respectively. The levels of HBV pgRNA and HBV DNA in these three groups were low concentration grouP<medium concentration grouP<high concentration group, P<0.05. Correlation analysis showed that HBV pgRNA was positively correlated with HBeAg (r=0.566, P=0.002), and HBV DNA was positively correlated with HBeAg (r=0.496, P=0.005).Conclusion With the increase of HBeAg level, HBV pgRNA and HBV DNA levels gradually increased. The serum HBV pgRNA and HBV DNA levels of CHB patients were positively correlated with HBeAg levels. Clinical diagnosis of HBV infection and replication and the condition of CHB patients could be more accurate by combining these three indicators.

Key words: Chronic hepatitis B, Hepatitis B virus pregenomic RNA, Hepatitis B viral DNA, Hepatitis B e antigen

卢艳玲, 李小红, 陈海涛. 慢性乙型肝炎患者血清HBV pgRNA与HBV DNA和HBeAg水平变化关系研究[J]. 肝脏, 2022, 27(3): 302-304.

LU Yan-ling, LI Xiao-hong, CHEN Hai-tao. A study on the relationship between serum HBV pgRNA, HBV DNA and HBeAg levels in patients with chronic hepatitis B[J]. Chinese Hepatolgy, 2022, 27(3): 302-304.

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