中性粒细胞胞外诱捕网在急性肝衰竭小鼠中的作用

肝脏 ›› 2025, Vol. 30 ›› Issue (1): 42-45.

中性粒细胞胞外诱捕网在急性肝衰竭小鼠中的作用

杨世欣, 石春霞, 郭金, 张丹眉, 王钰鲲, 龚作炯

430060 湖北武汉大学人民医院感染科

收稿日期:2024-04-22 出版日期:2025-01-31 发布日期:2025-03-10
通讯作者: 龚作炯,Email: zjgong@163.com
基金资助:
国家自然科学基金资助项目(82270627)

The effect of Neutrophil extracellular traps on mice with acute liver failure

YANG Shi-xin, SHI Chun-xia, GUO Jin, ZHANG, Dan-mei, WANG Yu-kun, GONG Zuo-jiong

Department of Infection, Renmin Hospital of Wuhan University, Wuhan 430060, China

Received:2024-04-22 Online:2025-01-31 Published:2025-03-10
Contact: GONG Zuo-jiong,Email: zjgong@163.com

摘要/Abstract

摘要： 目的分析急性肝衰竭(acute liver failure, ALF)小鼠肝组织中性粒细胞胞外诱捕网(neutrophil extracellular traps,NET)水平的变化,探讨NET在ALF肝脏炎症反应过程中的致病作用。方法将20只雄性小鼠随机分为正常组和模型组,每组10只。用D-氨基半乳糖联合脂多糖诱导小鼠急性肝衰竭模型,检测两组小鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)和总胆红素素(TBil)水平。原位缺口末端标记法检测肝细胞凋亡情况,免疫荧光检测肝脏中NET标志物瓜氨酸组蛋白H3的表达。采用酶联免疫吸附实验检测小鼠血清及肝脏组织中的NET的水平。结果 ALF模型组小鼠肝组织结构破坏,炎性细胞浸润明显增多;血清AST、ALT、TBil水平显著升高( P<0.05 )。模型组小鼠肝脏组织中NET为(10.52±0.53)ng/mL,高于正常组小鼠的(4.61±0.84) ng/mL,差异有统计学意义(P<0.05)。结论 NET在ALF期间的炎症反应中起重要作用。

关键词: 急性肝衰竭, 中性粒细胞胞外诱捕网, 瓜氨酸组蛋白H3

Abstract: Objective To analyze the changes of Neutrophil extracellular traps (NETs) in liver tissue of mice with acute liver failure (ALF). To investigate the pathogenic role of NETosis in ALF liver inflammation. Methods 20 male mice were randomly divided into a normal group and a model group with 10 mice in each group. Acute hepatic failure model in mice was induced with D-galactosamine in combination with lipopolysaccharide. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bilirubin (TBil) levels were detected in both groups of Mice. Hematoxylin-eosin (HE) staining was used to observe the pathological changes in liver. Terminal deoxynucleotidyl transferase dUTP Nick-End Labeling (TUNEL) staining was used to detect hepatocyte apoptosis. Immunofluorescence staining was used to detect the expression of citline histone H3, a marker of NETs in liver. Enzyme linked immunosorbent assay (ELISA) was used to detect the level of NETs in serum and liver tissue of mice. Methods The liver structure of ALF model group was damaged, and inflammatory cell infiltration increased significantly. Serum AST, ALT and TBil levels were significantly increased (P＜0.05) . NETs in the liver tissue of mice in model group (10.52±0.53 ng/mL) were significantly higher than those in normal mice (4.61±0.84 ng/mL), and the difference was statistically significant (P<0.05). Conclusion NETs play an important role in the inflammatory response of ALF. It can be used as a feasible target for the treatment of acute liver failure.

Key words: Acute liver failure, Neutrophil extracellular traps, Citrullinated histone H3

杨世欣, 石春霞, 郭金, 张丹眉, 王钰鲲, 龚作炯. 中性粒细胞胞外诱捕网在急性肝衰竭小鼠中的作用[J]. 肝脏, 2025, 30(1): 42-45.

YANG Shi-xin, SHI Chun-xia, GUO Jin, ZHANG, Dan-mei, WANG Yu-kun, GONG Zuo-jiong. The effect of Neutrophil extracellular traps on mice with acute liver failure[J]. Chinese Hepatolgy, 2025, 30(1): 42-45.

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