琥珀酸缓解小鼠高脂饮食所致肥胖及脂肪肝的作用

摘要/Abstract

摘要： 目的探讨琥珀酸在高脂饮食所致小鼠体重增加,血脂、血糖升高和并发脂肪肝中的缓解作用。方法采用区组随机法将30只小鼠分为对照组、高脂饲料组、高脂饲料+琥珀酸组,每组10只。称重各组小鼠初始体重及每干预1周后均再次称重1次,并进行组间比较;饲养11周后各组小鼠取血检测并比较血清总胆固醇(TC)、甘油三酯(TG)、血糖(GLU)、天冬氨酸氨基转移酶(AST)水平;处死各组小鼠取肝脏称重,计算出肝脏指数并进行比较;称重各组小鼠的肠系膜脂肪重量;取各组小鼠的肝脏组织采用苏木精-伊红染色法(HE)染色,镜下观察肝细胞形态,比较各组小鼠并发脂肪肝严重程度。结果与对照组比较,高脂饲料组和高脂饲料+琥珀酸组小鼠在饲养1周后体重均明显增加(P值均<0.05);饲养第2周、第3周后高脂饲料组和高脂饲料+琥珀酸组小鼠较对照组体重均明显增加(P值均<0.05),而高脂饲料+琥珀酸组小鼠体重要明显低于高脂饲料组(P值<0.05);饲养第4周到第11周后,高脂饲料组小鼠较对照组体重均明显增加(P值均<0.05),高脂饲料+琥珀酸组小鼠体重要明显低于高脂饲料组(P值均<0.05)。11周后,高脂饲料组与对照组比较,小鼠血清TC、TG、GLU、AST水平均升高(P值均<0.05),而高脂饲料+琥珀酸组小鼠血清TC、TG、GLU、AST水平均明显低于高脂饲料组(P值均<0.05)。高脂饲料组小鼠与对照组比较,肝脏指数及肠系膜脂肪重量均明显增加(P值均<0.05),而高脂饲料+琥珀酸组小鼠肝脏指数及肠系膜脂肪重量均明显低于高脂饲料组(P值均<0.05)。HE染色显示高脂饲料组小鼠肝脏细胞发生明显脂肪变性,而高脂饲料+琥珀酸组小鼠肝脏细胞脂肪变性要较高脂饲料组小鼠明显减轻。结论琥珀酸能明显缓解高脂饮食所致小鼠的体重增加,降低高脂饮食小鼠的TC、TG、GLU、AST水平及具有改善脂肪肝的作用。

关键词: 琥珀酸, 高脂饮食, 肥胖, 脂肪肝

Abstract: Objective To explore the alleviative effect of succinic acid on weight gain, elevated blood lipids and blood sugar, and concurrent hepatic steatosis in mice induced by the high-fat diet. Methods Thirty mice were divided into control group, high-fat diet group and succinic acid-treated high-fat diet group with 10 mice in each group by block random method. The body weight of mice in each group was measured at the beginning of the experiment and subsequently every week. Comparisons between groups were carried out. After 11 weeks of feeding, the levels of serum total cholesterol (TC), triglyceride (TG), blood glucose (GLU) and aspartate transaminase (AST) were measured and compared between each group. Mice were sacrificed and livers were weighed to calculate the liver index. The mesenteric fat of each group was also weighed. The livers of mice in each group were stained with hematoxylin-eosin (HE) method, the morphology of liver cells was observed under the microscope to assess the severity of fatty liver. Results Compared with the control group, the body weight of mice in high-fat diet group and the succinic acid-treated high-fat diet group significantly increased after 1 week of feeding (all P<0.05). After 2 and 3 weeks of feeding, the body weight of mice in high-fat diet group and the succinic acid-treated high-fat diet group mice were significantly increased compared with that of control group (all P<0.05). However, the body weight of mice in the succinic acid-treated high-fat diet group were significantly lower than that in the high-fat diet group (P<0.05). After the 4th week to the 11th week, the body weight of mice in the high-fat diet group were significantly higher than that in the control group (all P<0.05), and the body weight of the mice in succinic acid-treated high-fat diet group were significantly lower than that in the high-fat diet group (all P<0.05). After 11 weeks of feeding, the levels of serum TC, TG, GLU and AST in the high-fat diet group mice were increased significantly compared with the control group (all P<0.05). The levels of serum TC, TG, GLU and AST in the succinic acid-treated high-fat diet group were significantly lower than those in the high-fat diet group (all P<0.05). The liver index and mesenteric fat weight in the high-fat diet group were significantly increased compared with the control group (all P<0.05), and the liver index and mesenteric fat weight of mice in the succinic acid-treated high-fat diet group were significantly lower than those in the high-fat diet group (all P<0.05). HE staining showed that the liver cells of mice in the high-fat diet group had obvious steatosis, while the liver cells steatosis of mice in the succinic acid-treated high-fat diet group were significantly alleviated compared with that in the high-fat diet group. Conclusion The succinic acid can significantly alleviate the weight gain caused by high-fat diet in mice, reduce the levels of TC, TG, GLU, AST in the high-fat diet mice, and alleviate fatty liver disease.

Key words: Succinic acid, High-fat diet, Obesity, Fatty liver

徐忆青, 陈园, 崔文琪, 张学敏. 琥珀酸缓解小鼠高脂饮食所致肥胖及脂肪肝的作用[J]. 肝脏, 2025, 30(2): 236-239.

XU Yi-qing, CHEN Yuan, CUI Wen-qi, ZHANG Xue-min. Succinic acid alleviates obesity and fatty liver disease induced by high fat diet in mice[J]. Chinese Hepatolgy, 2025, 30(2): 236-239.

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