儿童EB病毒感染相关肝损伤患者外周血CD4+、CD8+T淋巴细胞功能分析

摘要/Abstract

摘要： 目的评价儿童EB病毒感染相关肝损伤患者外周血CD4⁺、CD8⁺T淋巴细胞功能。方法选取2022年1月至2024年6月于本院诊治的儿童EB病毒感染者104例,根据是否合并肝损伤分为肝损伤组、无肝损伤组,比较两组一般资料、治疗前后外周血CD4⁺、CD8⁺T淋巴细胞亚群及其表面标记物表达水平。结果 104例患儿中肝损伤、无肝损伤分别为43例、61例,肝损伤组ALT、AST、TBil水平为(56.3±17.3)U/L、(50.2±15.5)U/L、(23.2±4.3)μmol/L,均分别显著高于无肝损伤组[(35.6±11.0)U/L、(35.1±9.7)U/L、(15.2±2.4)μmol/L,P<0.05]。治疗后肝损伤组CD4⁺T淋巴细胞比例、CD4⁺/CD8⁺为(36.7±3.6)%、(1.2±0.2),均分别显著高于无肝损伤组[(34.7±3.2)%、(1.1±0.4),P<0.05]。肝损伤组外周血CD4⁺T淋巴细胞表面CD45RA、CD45RO、CCR3及CD28表达百分比为21.2(16.3,24.4)%、19.0(14.5,23.2)%、15.1(8.9,23.8)%及42.3(36.2,52.7)%,均分别显著高于无肝损伤组[18.2(14.3,23.5)%、13.7(9.3,17.2)%、8.1(4.0,10.3)%及33.6(30.3,38.4)%,P<0.05];肝损伤组外周血CD8⁺T淋巴细胞表面CD45RA、CCR3及CD25表达百分比为17.3(13.4,19.7)%、1.1(0.5,2.6)%及0.6(0.3,1.0)%,均分别显著高于无肝损伤组[13.9(9.6,17.1)%、0.7(0.4,0.9)%及0.3(0.2,0.4)%](P<0.05)。结论合并肝损伤的EB病毒感染患儿外周血CD4⁺、CD8⁺细胞比例失衡及其细胞表面标记物的异常表达,可能对患儿的临床结局产生影响,值得进一步研究。

关键词: EB病毒感染, 肝损伤, 淋巴细胞功能, 免疫功能

Abstract: Objective To evaluate the function of CD4⁺ and CD8⁺ T lymphocytes in peripheral blood of children with EB virus infection-related liver damage. Methods 104 cases of children infected with EB virus between January 2022 and June 2024 in our hospital were divided into liver damage group and non-liver damage group according to whether they were complicated with liver damage or not. The general data, CD4⁺, CD8⁺ T lymphocyte subsets and their surface markers expression levels in peripheral blood of the two groups were compared before and after treatment. Results There were 43 cases with liver damage and 61 cases without liver damage in 104 children. The levels of ALT, AST and TBil in the liver damage group were (56.3±17.3) U/L, (50.2±15.5) U/L and (23.2±4.3) μmol/L, which were significantly higher than those in the non-liver damage group [(35.6±11.0) U/L, (35.1±9.7) U/L, (15.2±2.4) μmol/L, P<0.05]. After treatment, the ratio of CD4⁺ T lymphocytes and CD4⁺/CD8⁺ in the liver damage group were (36.7±3.6)% and (1.2±0.2), which were significantly higher than those in the non-liver damage group [(34.7±3.2)% and (1.1±0.4), respectively, P<0.05]. The expression percentages of CD45RA, CD45RO, CCR3 and CD28 on the surface of CD4⁺ T lymphocytes in liver damage group were 21.2 (16.3, 24.4)%, 19.0 (14.5, 23.2)%, 15.1 (8.9, 23.8)% and 42.3 (36.2, 52.7)%, they were significantly higher than those in the non-liver damage group [18.2 (14.3, 23.5)%, 13.7 (9.3, 17.2)%, 8.1 (4.0, 10.3)% and 33.6 (30.3, 38.4)%, P<0.05]. The expression percentages of CD45RA, CCR3 and CD25 on the surface of CD8⁺ T lymphocytes in liver damage group were 17.3 (13.4, 19.7)%, 1.1 (0.5, 2.6)% and 0.6 (0.3, 1.0)%, they were significantly higher than those in the non-liver damage group [13.9 (9.6, 17.1)%, 0.7 (0.4, 0.9)% and 0.3 (0.2, 0.4)%] (P<0.05). Conclusion The imbalance of CD4⁺ and CD8⁺ cells in peripheral blood and the abnormal expression of cell surface markers in children with EB virus infection complicated with liver damage may affect the clinical outcome of patients, whichdeserves further study.

Key words: EB virus infection, Liver damage, Lymphocyte function, Immunologic function

蔡欣, 陈正徐, 袁润林, 柴烨. 儿童EB病毒感染相关肝损伤患者外周血CD4⁺、CD8⁺T淋巴细胞功能分析[J]. 肝脏, 2025, 30(5): 705-708.

CAI Xin, CHEN Zheng-xu, YUAN Run-lin, CHAI Ye. Function analysis of CD4⁺ and CD8⁺T lymphocytes in peripheral blood of children with EB virus infection-related liver damage[J]. Chinese Hepatolgy, 2025, 30(5): 705-708.

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