对乙酰氨基酚肝损伤时炎性因子、趋化因子以及Toll样受体表达的动态变化

肝脏 ›› 2016, Vol. 21 ›› Issue (1): 24-27.

对乙酰氨基酚肝损伤时炎性因子、趋化因子以及Toll样受体表达的动态变化

金宇霆, 李大伟, 明雅南, 张江, 张明, 夏强

200127 上海交通大学医学院附属仁济医院肝脏外科（金宇霆,李大伟,张江,张明,夏强）; 消化内科（明雅南）

收稿日期:2015-08-31 发布日期:2020-06-01
通讯作者: 夏强,Email：xiaqiang@med mail.com.cn
基金资助:
国家自然基金项目（81472243）; 上海市卫生局重点联合攻关项目（2013ZYJB001）

Dynamic variation of inflam matory cytokines,chemokines and Toll-like receptors in APAP-induced liver injury

JIN Yu-ting, LI Da-wei, MING Ya-nan, ZHANG Jiang, ZHANG Ming, XIA Qiang

Department of Liver Surgery,RenJi Hospital,ShanghaiJiao Tong University School of Medicine,Shanghai 200127,China

Received:2015-08-31 Published:2020-06-01
Contact: XIAQiang,Email：xiaqiang@medmail.com.cn

摘要/Abstract

摘要： 目的研究对乙酰氨基酚（APAP）引起肝脏组织损伤过程中的炎性因子、趋化因子以及Toll样受体的动态变化。方法随机将30只小鼠分为6组：对照组、APAP1 h组、APAP3 h组、APAP6 h组、APAP12 h组,APAP24 h组,每组5只。禁食15 h后,对照组小鼠腹腔注射0.9％氯化钠溶液,其余5组腹腔注射APAP300 Mg/kg诱发小鼠肝损伤。取各组小鼠肝脏组织采用实时PCR检测炎性因子、趋化因子以及Toll样受体的MRNA水平,HE染色观察各组肝脏组织损伤情况。结果与正常对照组相比,24 h内APAP诱发的小鼠肝损伤随时间推移加重。IL-6、IL-10、IL-1β、IP-10 MRNA相对于对照组在不同时间点有不同程度升高;MIP-3α 24 h相较对照组明显下降（P<0.05）;IFN-γ和TNF-α各时间点变化差异无统计学意义。趋化因CXCR-2和CCR-2在24 h与对照组比较明显下降（P<0.05）;CXCL-2分别在12 h 和24 h升高14.8倍（P<0.05）和7.8倍（P<0.05）。Toll样受体TLR-9、TLR-4相对对照组在不同时间点亦有不同程度升高或降低。结论对乙酰氨基酚诱导肝损伤后,肝组织炎性因子、趋化因子及Toll样受体MRNA表达水平发生明显改变。

关键词: 对乙酰氨基酚, 炎症因子, 趋化因子, Toll样受体

Abstract: Objective To explore the dynamic changes ofinflam matory cytokines,chem okines and Toll-like receptors （TLR）in acetaminophen（APAP）-induced liver injury within 24 hours. Methods C57BL/6 mice were rando mly divided into six groups：control group,APAP1h group,APAP3h group,APAP6h group,APAP12h group and APAP24h group,including 5 mice in each group. After fasting for 15 hours,control group were given 0.9％saline by intraperitoneal injection,w hile other groups were given 300 Mg/kg APAP. Assessment of the various inflam matory cytokines,chem okines and TLRin mice liver was carried out at MRNAlevel by realtime poly merase chain reaction（RT-PCR）. Degrees of APAP-induced liver injury were evaluated by haematoxylin eosin（HE）staining. Results According to HEstaining result,APAP-induced liver injury in APAPgroups increasingly aggravated within 24 hours w hen compared with that in control group.In APAPgroups,RT-PCRindicated that MRNAlevels of IL-6,IL-10,IL-1βand IP-10 rose to various degrees at different time points,w hile MIP-3α MRNAlevels declined distinctly（P<0.05）,which was in contrast to control group. Additionally,variation ofinterferon（IFN）-γ and tu Mor necrosis factor（TNF）-α had no statistical difference between APAPand control groups.In APAPgroups,levels of CXCR-2 and CCR-2 were obviously lower at 24h（P<0.05）than those in control group,w hile level of CXCL-2 rose to 14.8 and 7.8 times higher at 12h and 24h（P<0.05）,respectively. Furtherm ore,levels of TLR-9 and TLR-4 changed to various extents at different time points（P<0.05）. Conclusion APAPfor 300 Mg/kg induces mice liver injury within 24h,which makes inflam matory cytokines,chem okines and TLRchange obviously at MRNAlevels in mice liver.

Key words: Acetaminophen, Cytokine, Chem okine, Toll-like receptor

金宇霆, 李大伟, 明雅南, 张江, 张明, 夏强. 对乙酰氨基酚肝损伤时炎性因子、趋化因子以及Toll样受体表达的动态变化[J]. 肝脏, 2016, 21(1): 24-27.

JIN Yu-ting, LI Da-wei, MING Ya-nan, ZHANG Jiang, ZHANG Ming, XIA Qiang. Dynamic variation of inflam matory cytokines,chemokines and Toll-like receptors in APAP-induced liver injury[J]. Chinese Hepatolgy, 2016, 21(1): 24-27.

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