自身免疫性肝炎患者肝组织程序性细胞死亡因子1及其配体1的表达及其与临床的相关性

肝脏 ›› 2016, Vol. 21 ›› Issue (11): 911-914.

自身免疫性肝炎患者肝组织程序性细胞死亡因子1及其配体1的表达及其与临床的相关性

柯坤宇, 魏大海, 陈丽芳, 孔金峰, 孙芳, 熊克宫

350025 福州福州市传染病医院(福建医科大学附属传染病医院)肝病区

收稿日期:2016-06-28 出版日期:2016-11-30 发布日期:2020-06-12
通讯作者: 熊克宫,Email:510768115@qq.com
基金资助:
福州市卫生系统科技计划项目(2014-S-w21)

Expressions of PD and PD-L 1 in liver tissues of patients with autoimmune hepatitis and their clinic correlation

KE Kun-yu, WEI Da-hai, CHEN Li-fang, KONG Jin-feng, SUN Fang, XIONG Ke-gong

Department of liver, The infectious disease hospital of Fujian medical university, Fuzhou 350012, China

Received:2016-06-28 Online:2016-11-30 Published:2020-06-12
Contact: XIONG Ke-gong, Emal: 510768115@qq.com

摘要/Abstract

摘要： 目的通过检测程序性细胞死亡因子1(PD-1)及其配体1(PD-L1)在自身免疫性肝炎(AIH))患者不同分期肝组织中的表达情况,探讨其在AIH发病机制中的作用。方法收集43例AIH患者(包括活动期31例和缓解期12例)肝组织,并选择9例健康者为对照组。应用免疫组织化学方法结合图像定量分析系统检测肝组织内PD-1及PD-L1的表达情况,并比较不同病情分期PD-1及PD-L1表达的差异。结果 AIH患者肝组织PD-1为(10.12±5.31)%,高于对照组(1.74±0.37)%,(P<0.05),AIH活动期肝组织PD-1为(15.68±6.31)%,高于缓解期(6.24±3.25)%,(P<0.05)。AIH患者肝组织PD-L1为(7.45±4.29)%,高于对照组(1.48±0.32)%,(P<0.05);AIH活动期肝组织PD-L1为(9.47±4.67)%,高于缓解期(4.78±2.43)%(均P<0.05)。结论 PD-1及其配体PD-L1在AIH患者肝组织中高表达,提示其可能参与了AIH的发病机制。

关键词: AIH, PD-1, PD-L1, 肝组织, 免疫组织化学

Abstract: Objective To investigate the expressions of programmed death 1 (PD-1) and programmed death ligand 1 (PD-L1) in hepatic tissues of patients with autoimmune hepatitis (AIH), and to explore their roles in the pathogenesis of AIH. Methods The expressions of PD-1 and PD-L1 in hepatic tissues from 43 AIH patients and 9 healthy controls were detected by immunohistochemistry combined with computer image quantitative analysis. Results The PD-1 and PD-L1 expressions in hepatic tissues of AIH patients were 10.12±5.31% and 7.45±4.29%, respectively, which were significantly higher than those in controls (1.74±0.37% and 1.48±0.32%, respectively). The expressions of PD-1 and PD-L1 in hepatic tissues of AIH patients at active stage were 15.68±6.31% and 7.45±4.29%, respectively, which were higher than those in AIH patients in remission (6.24±3.25% and 4.78±2.43%, respectively) and healthy controls (1.74±0.37% and 1.48±0.32%, respectively). Conclusion PD-1 and PD-L1 are overexpressed in hepatic tissues of AIH patients, which reveals that PD-1 and its ligand might play an important role in the pathogenesis of AIH.

Key words: AIH, PD-1, PD-L1, Liver tissues, Immunohistochemistry

柯坤宇, 魏大海, 陈丽芳, 孔金峰, 孙芳, 熊克宫. 自身免疫性肝炎患者肝组织程序性细胞死亡因子1及其配体1的表达及其与临床的相关性[J]. 肝脏, 2016, 21(11): 911-914.

KE Kun-yu, WEI Da-hai, CHEN Li-fang, KONG Jin-feng, SUN Fang, XIONG Ke-gong. Expressions of PD and PD-L 1 in liver tissues of patients with autoimmune hepatitis and their clinic correlation[J]. Chinese Hepatolgy, 2016, 21(11): 911-914.

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