HBx及Beclin1在肝癌中表达的关系研究

摘要/Abstract

摘要： 目的了解HBx和Beclin1在原发性肝癌、癌旁组织的表达水平,及其在HCC的发病机制中作用及其相互关系。方法收集经病理确诊的HCC患者64例,患者术前未经任何抗肿瘤治疗,采用免疫组化、Western印迹及实时PCR方法检测HBx及Beclin l在肝癌组织及其癌旁组织中的表达情况,并将实验结果与患者的临床资料结合进行统计学分析。结果 53例患者的肝癌组织HBx蛋白表达量高于癌旁组织,差异有统计学意义（P<0.05）;肝癌组织与癌旁组织中HBx与GAPDH相对灰度比值分别为1.54±0.13、0.62±0.07,肝癌组织表达量明显高于癌旁组织,差异有统计学意义（P<0.05）、肝癌组织与癌旁组织中 HBx表达量分别为0.78±0.10、0.11±0.01,差异有统计学意义（P<0.05）。51例患者肝癌组织Beclin1蛋白表达量高于癌旁组织,差异有统计学意义（P<0.05）。肝癌组织与癌旁组织中Beclin1与GAPDH相对灰度比值分别为1.52±0.11、0.98±0.05,肝癌组织表达量明显高于癌旁组织,差异有统计学意义（P<0.05）。肝癌组织与癌旁组织中Beclin1表达量分别为0.83±0.15、0.18±0.03,差异有统计学意义（P<0.05）。结论 HBx和 Beclin1在HCC中高表达,两者相互作用且共同参与HCC的发生、发展过程,联合检测 HBx和Beclin1对判断HCC的发生和转移有重要价值,是有潜力的肝癌治疗的候选靶基因。

关键词: HBx, Beclin1, 肝细胞癌, 聚合酶链式反应, 蛋白质印迹法, 免疫组化

Abstract: Objective To investigate the specific role of hepatitis B virus X protein （HBx）and Beclin1 protein（Beclin1）in the pathogenesis of hepatocellular carcinoma（HCC）and their correlations.Methods Carcinoma tissue and adjacent tissue from 64 patients pathologically diagnosed as HCC without any treatment before surgery were collected in our hospital from January 2014 to December 2015.The expressions of HBx and Beclin l in carcinoma tissue and adjacent tissue of all cases were detected by real-time polymerase chain reaction（PCR）,western blot and immunohistochemistry（IHC）,which were analyzed in detail combined with their clinical data.Results Compared to those in adjacent tissue,higher levels of HBx and Beclin1 were detected by IHC in 82.8%and 79.7%of HCC tissue,respectively.Meanwhile,both HBx and Beclin1 showed higher protein levels in HCC tissues than that in adjacent tissues by western blot,as well as m RNA levels（0.78±0.10 vs.0.11±0.01 and 0.83±0.15 vs.0.18±0.03）by real-time PCR,respectively.Conclusion HBx and Beclin1 are both overexpressed in HCC and might participate in the pathogenesis of HCC.Combined detection of HBx and Beclin1 might play an important role in prediction of occurrence and metastasis of HCC,which might be potential target genes for HCC treatment.

Key words: HBx, Beclin1, Hepatocellular carcinoma, Real time-PCR, Western blot, Immunohistochemistry

段昌虎, 刘晓晨, 豆发福, 丁建龙, 段建峰, 赵喜荣, 周亚东, 李光华, 杨帆. HBx及Beclin1在肝癌中表达的关系研究[J]. 肝脏, 2017, 22(1): 8-.

DUAN Chang-hu, LIU Xiaochen, DOU Fa-fu, DING Jian-long, DUAN Jian-feng, ZHAO Xi-rong, ZHOU Ya-dong, LI Guang-hua, YANG Fan. Exression and clinical sinificance of HBx and Beclin1 in heatocellular carcinoma[J]. Chinese Hepatolgy, 2017, 22(1): 8-.

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