关键词: 骨髓间充质干细胞, ALF, Bcl-2基因, 细胞示踪

Abstract: Objective To construct Bcl-2 gene-modified lentivirus,establish stable expression of Bcl-2 gene in bone marrow mesenchymal stem cell lines,and discuss the feasibility of bone marrow Bcl-2 gene-modified mesenchymal stem cells therapy in acute liver failure（ALF）to the problems of liver apoptosis and regeneration.Methods Bcl-2 gene fragment synthesized by polymerase chain reaction（PCR）was inserted into GV287 empty vector to be amplified and packaged in lentivirus.Bcl-2 lentivirus were transfected into rat bone marrow mesenchymal stem cells.Then bone marrow mesenchymal stem cells were transplanted into rats with acute liver injury,and survival rates and liver pathology were observed after transplantation.Results The purified bone marrow mesenchymal stem cells lines were obtained.Bcl-2 gene fragments were separated for construction of Bcl-2 gene lentivirus,which was transfected into bone marrow mesenchymal stem cells successfully.Through detecting by green fluorescent protein（GFP）fluorescence and western blot,transfected cells were in good condition with high expression of Bcl-2.Rat models were transplanted with bone marrow mesenchymal stem cells through tail vein,and PKH26 fluorescence-labeled bone marrow mesenchymal stem cells can be detected in damaged liver tissue by liver pathology frozen section after transplantation.Conclusion We preliminarily confirm that bone marrow mesenchymal stem cells can home to liver tissue in rats with acute liver injury with certain therapeutic effects.

Key words: Mesenchymal stem cells, Acute liver failure, Bcl-2 gene, Cell tracking