细胞自噬及相关因子在老年肝外伤中的表达及意义

肝脏 ›› 2016, Vol. 21 ›› Issue (7): 576-579.

细胞自噬及相关因子在老年肝外伤中的表达及意义

滕旭, 许赤, 孟炜, 李晓华

518117 广东广东省深圳市龙岗区第六人民医院普外科(滕旭);中山大学附属三院肝外科(许赤,孟炜,李晓华)

收稿日期:2016-02-20 出版日期:2016-07-31 发布日期:2020-07-09

Received:2016-02-20 Online:2016-07-31 Published:2020-07-09

摘要/Abstract

摘要： 目的研究细胞自噬及相关因子在老年肝外伤中的表达,探讨在老年肝脏缺血再灌注损伤中诱导老年肝细胞自噬的分子机制。方法选取不同鼠龄的大鼠,建立老年大鼠肝外伤缺血再灌注损伤的模型,通过采用电子显微镜、免疫印迹和荧光显微镜等方法观察细胞自噬与细胞凋亡,研究因子Bcl-2及Beclin 1在细胞自噬/细胞凋亡之间的调控关系。结果 Beclin 1在老年大鼠肝外伤再灌注期表达明显增多,而Bcl-2的表达下调(P<0.01), Bcl-2和Beclin 1平衡状态失调,免疫印迹表明Beclin 1阳性细胞百分率(40.8±10.6)%与Bcl-2(17.6±5.2)%比较差异有统计学意义(P<0.01),而在中晚期阶段(外伤后3~6月),Bcl-2表达下调的作用减缓,同时也激活Beclin 1激活自噬,此时年轻大鼠与老年大鼠的肝功能检测及病理切片检查差异不大(P>0.01)。结论 Bcl-2与Beclin 1在老年肝脏缺血再灌注损伤的分子机制中可能发挥着重要作用。

滕旭, 许赤, 孟炜, 李晓华. 细胞自噬及相关因子在老年肝外伤中的表达及意义[J]. 肝脏, 2016, 21(7): 576-579.

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