SIRT1通过增强分子伴侣介导的自噬降低细胞内脂质堆积改善非酒精性脂肪肝

肝脏 ›› 2020, Vol. 25 ›› Issue (12): 1320-1322.

SIRT1通过增强分子伴侣介导的自噬降低细胞内脂质堆积改善非酒精性脂肪肝

马颖, 马玲, 马明

830054 乌鲁木齐新疆医科大学第一附属医院医学检验中心(马颖);新疆维吾尔自治区胸科医院(马玲);新疆维吾尔自治区人民医院肝胆外科(马明)

收稿日期:2020-04-03 出版日期:2020-12-31 发布日期:2021-02-26
通讯作者: 马明
基金资助:
新疆维吾尔自治区自然科学基金资助项目(2018D01C186)

SIRT1 improves nonalcoholic fatty liver disease by enhancing chaperone mediated autophagy (CMA) and reducing intracellular lipid accumulation

MA Ying¹, MA Ling², MA Ming^3*

1. The First Affiliated Hospital of Xinjiang Medical University, Wulumuqi 830054,China;
2. Chest Hospital of Xinjiang Uygur Autonomous Region, Wulumuqi 830001, China;
3. Xinjiang Uygur Autonomous Region People's Hospital, Wulumuqi 830001, China

Received:2020-04-03 Online:2020-12-31 Published:2021-02-26
Contact: MA Ming

摘要/Abstract

摘要： 目的探讨分子伴侣诱导的自噬在SIRT1 改善非酒精性脂肪肝病理发展中的作用。方法验证非酒精性脂肪肝模型下SIRT1和自噬水平的变化,通过在小鼠正常肝脏细胞过表达SIRT1并使用棕榈酸油酸24 h造模后,检测脂肪肝模型下细胞内三酰甘油的变化情况和自噬水平的变化,通过siRNA下调SIRT1,检测脂肪肝模型下细胞内三酰甘油的变化情况和自噬水平的变化。结果 SIRT1的过表达组与对照组相比较,细胞内甘油三酯显著降低,肝脏细胞内分子伴侣诱导的自噬水平上调。而SIRT1沉默组与对照组比较,细胞内三酰甘油明显升高,肝脏细胞内分子伴侣诱导的自噬水平下调。结论 SIRT1 可能通过上调分子伴侣诱导的自噬来减少肝细胞中的脂肪堆积,从而改善脂肪肝。

关键词: 自噬 SIRT1 非酒精性脂肪肝

Abstract: Objective To investigate the role of molecular chaperone induced autophagy in the improvement of SIRT1 in the pathological development of nonalcoholic fatty liver disease.Methods Validation SIRT1 under the model of nonalcoholic fatty liver disease and the change of the level of autophagy. In normal liver cells of mice by SIRT1 over expression and useing of palmitic acid and oleic acid for 24 h, detecting the changes of the intracellular triglyceride (TG) and the level of autophagy. Downregulate SIRT1 by siRNA, detecting the change of intracellular triglyceride (TG) and the level of autophagy.Results Compared with the control group, the over-expression group of SIRT1 significantly reduced intracellular triglyceride, and the level of autophagy induced by molecular chaperone in liver cells was up-regulated. Comparing with the control group, the SIRT1 silencing group showed significantly increased intracellular triglycerides and down-regulated autophagy induced by molecular chaperone in liver cells.Conclusion SIRT1 may improve fatty liver disease, by upregulating molecular chaperone-induced autophagy to reduce fat accumulation in hepatocytes.

Key words: Autophagy, SIRT1, non-alcoholic fatty liver disease

马颖, 马玲, 马明. SIRT1通过增强分子伴侣介导的自噬降低细胞内脂质堆积改善非酒精性脂肪肝[J]. 肝脏, 2020, 25(12): 1320-1322.

MA Ying, MA Ling, MA Ming. SIRT1 improves nonalcoholic fatty liver disease by enhancing chaperone mediated autophagy (CMA) and reducing intracellular lipid accumulation[J]. Chinese Hepatolgy, 2020, 25(12): 1320-1322.

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