妊娠期肝内胆汁淤积症蜕膜组织T细胞和B细胞失调与病情程度的关系

肝脏 ›› 2020, Vol. 25 ›› Issue (12): 1323-1325.

妊娠期肝内胆汁淤积症蜕膜组织T细胞和B细胞失调与病情程度的关系

王吕, 王惠, 汤卫春, 陆月梅

226600 江苏南通大学附属海安医院妇产科(王吕,王惠);南通市第一人民医院(汤卫春);海安人民医院妇产科(陆月梅)

收稿日期:2020-06-28 出版日期:2020-12-31 发布日期:2021-02-26
通讯作者: 王惠,Email:2757822@qq.com
基金资助:
南通市科技项目(GJZ16017)

Relationship between dysregulation of T and B cells in decidual tissue of intrahepatic cholestasis of pregnancy and the degree of disease

WANG Lv¹, WANG Hui¹, TANG Wei-chun², LU Yue-mei³

1. Department of Obstetrics and Gynecology, Hai'an Hospital Affiliated to Nantong University, Jiangsu 226600, China;
2. Department of Obstetrics and Gynecology, The First People's Hospital of Nantong, Jiangsu 226001, China;
3. Department of Obstetrics and Gynecology , Hai'an People's Hospital, Jiangsu 226600, China

Received:2020-06-28 Online:2020-12-31 Published:2021-02-26
Contact: WANG Hui,Email:2757822@qq.com

摘要/Abstract

摘要： 目的探讨妊娠期肝内胆汁淤积症(ICP)蜕膜组织中T细胞、B细胞失调与病情程度的关系。方法 2017年10月至2019年10月南通大学附属海安医院收治的ICP孕妇116例和同期健康体检孕妇110例,采用流式细胞仪检测蜕膜组织中T细胞亚型及B细胞含量。分析不同病情程度ICP患者蜕膜组织中T细胞、B细胞占蜕膜单个核细胞的百分率,并采用受试者操作特征曲线(ROC)评价蜕膜中T、B细胞占其蜕膜单个核细胞的百分率对ICP的诊断效能。结果 ICP组蜕膜组织中CD3⁺、CD3⁺CD4⁺、CD4⁺CD25⁺和CD19⁺占蜕膜单个核细胞的百分率分别为(46.3±4.0)%、(19.2±4.2)%、(6.4±2.0)%和(1.2±0.2)%,显著低于对照组[分别为(54.0±3.0)%、(25.6±5.0)%、(14.5±3.2)%和(3.2±0.6)%,P<0.05]。116例ICP患者中,轻度86例(74.1%),重度30例(25.9%)。轻度ICP者蜕膜组织中CD3⁺、CD3⁺CD4⁺、CD4⁺CD25⁺和CD19⁺占其蜕膜单个核细胞的百分率分别为(47.8±4.3)%、(20.4±4.0)%、(6.6±1.3)%和(1.3±0.5)%,显著高于重度ICP者[分别为(42.0±2.8)%、(15.6±4.5)%、(6.0±1.4)%和(0.9±0.3)%,P<0.05]。经ROC曲线处理,结果显示蜕膜组织中CD3⁺、CD3⁺CD4⁺、CD4⁺CD25⁺、CD19⁺占蜕膜单个核细胞的百分率对ICP有一定诊断价值,曲线下面积分别为0.777、0.744、0.823、0.870。结论蜕膜组织中T细胞、B细胞失调可能参与ICP的发病过程,且与病情严重程度密切相关。

关键词: 妊娠期肝内胆汁淤积症, 蜕膜组织, T细胞, B细胞

Abstract: Objective To explore the relationship between dysregulation of T and B cells in decidual tissue of intrahepatic cholestasis of pregnancy (ICP) during pregnancy and the degree of the disease.Methods One hundred and sixteen ICP pregnant women and 110 healthy physical examination pregnant women in our hospital between October 2017 and October 2019, and detection of T cell subtype and B cell content in decidual tissue by flow cytometry. The percentages of T and B cells in decidual mononuclear cells in ICP patients with different degrees of illness were analyzed, and the percentage of T and B cells in the decidual mononuclear cells in the decidual mononuclear cells was evaluated by ROC to evaluate the diagnostic efficacy of ICP.Results The percentages of CD3⁺, CD3⁺CD4⁺, CD4⁺CD25⁺ and CD19⁺ in the decidual tissue of ICP group accounted for (46.3±4.0)%, (19.2±4.2)%, (6.4±2.0)% and (1.2±0.2)%, significantly lower than the control group [respectively (54.0±3.0)%, (25.6±5.0)%, (14.5±3.2)% and (3.2±0.6)%, P<0.05].Among 116 patients with ICP, 86 were mild (74.1%) and 30 were severe (25.9%).The percentage of CD3⁺, CD3⁺CD4⁺, CD4⁺CD25⁺ and CD19⁺ in the decidual mononuclear cells of mild ICP was (47.8±4.3)%, (20.4±4.0)%, (6.6±1.3)% and (1.3±0.5)%, significantly higher than those with severe ICP [respectively (42.0±2.8)%, (15.6±4.5)%, (6.0±1.4)% and (0.9±0.3)%, P<0.05]. After ROC curve treatment, the results showed that the percentages of CD3⁺, CD3⁺CD4⁺, CD4⁺CD25⁺ and CD19⁺ in decidual mononucleocytes had certain diagnostic value for ICP, and the areas under the curve were 0.777, 0.744, 0.823 and 0.870, respectively.Conclusion The imbalance of T and B cells in decidua may be involved in the pathogenesis of ICP, and it is closely related to the severity of the disease.

Key words: Intrahepatic cholestasis of pregnancy, Decidual tissue, T cells, B cells

王吕, 王惠, 汤卫春, 陆月梅. 妊娠期肝内胆汁淤积症蜕膜组织T细胞和B细胞失调与病情程度的关系[J]. 肝脏, 2020, 25(12): 1323-1325.

WANG Lv, WANG Hui, TANG Wei-chun, LU Yue-mei. Relationship between dysregulation of T and B cells in decidual tissue of intrahepatic cholestasis of pregnancy and the degree of disease[J]. Chinese Hepatolgy, 2020, 25(12): 1323-1325.

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