GO-CoA-Tat对非酒精性脂肪性肝病小鼠的肝损伤保护作用

摘要/Abstract

摘要： 目的探讨GO-CoA-Tat对非酒精性脂肪性肝病小鼠肝损伤的保护作用。方法建立高脂饮食(HFD)脂肪肝小鼠模型。18只C57bl/6雄性小鼠分成3组:正常组、HFD+Saline组及HFD+GO-CoA-Tat组。HFD+Saline组及HFD+GO-CoA-Tat组。HFD喂养8周以诱导慢性NAFLD模型,分别单独皮下注射0.9% NaCl或GO-CoA-Tat。每周测量各组小鼠饲喂期间体质量变化。检测血清生化指标ALT、AST、TG、TC及GLU。细胞脂质测定试剂盒检测小鼠肝脏三酰甘油浓度。RT-PCR检测TNF-α和IL-6 mRNA表达变化。结果 HFD+GO-CoA-Tat组第8周体质量为(33.125±0.153)g,HFD+Saline组为(37.327±0.296)g,差异有统计学意义(P=0.014)。HFD+GO-CoA-Tat组TG及TC为(1.237±0.071)、(2.403±0.151)mmol/L,HFD+Saline组为(1.943±0.136)、(5.013±0.369)mmol/L,差异有统计学意义(P值分别为0.038和0.029)。HFD+GO-CoA-Tat组GLU(4.103±0.184)mmol/L,HFD+Saline组(6.370±0.296)mmol/L,差异有统计学意义(P=0.013)。HFD+GO-CoA-Tat组小鼠肝组织三酰甘油含量为(75.602±1.487)mmol/L,HFD+Saline组为(101.332±2.052)mmol/L,(P=0.027)。HFD+GO-CoA-Tat组ALT及AST为(37.862±2.425)、(122.932±6.907)U/L,HFD+Saline组为(54.901±5.201)、(173.321±13.971)U/L,差异有统计学意义(P值分别为0.035和0.026)。HFD+GO-CoA-Tat组TNF-α和IL-6为(1.730±0.040)、(1.630±0.040),HFD+Saline组为(2.740±0.140)、(2.980±0.070)差异有统计学意义(P值分别为0.042和0.038)。结论 GO-CoA-Tat对脂肪肝小鼠肝损伤具有保护作用。

关键词: 非酒精性脂肪性肝病, 高脂饮食, C57bl/6雄性小鼠, 饥饿素-O-酰基转移酶

Abstract: Objective To investigate the protective effect of GO-CoA-Tat, an antagonist of Ghrelin o-acyltransferase, on liver injury of mice with non-alcoholic fatty liver.disease (NAFLD).Methods High fat diet (HFD) induced mice model of fatty liver disease was established. C57BL/6 male mice were feed with HFD for 8 weeks to induce chronic NAFLD. The mice were separately injected with physiological saline or GO-COA-TAT subcutaneously. The experimental groups included a Normal group, an HFD+Saline group and an HFD+GO-CoA-Tat group. Mice weights during feeding period were measured weekly. Microplate detection kits were used for detecting alanine aminotransferase (ALT), aspartame aminotransferase (AST), triglyceride (TG), total cholesterol (TC) and glucose (GLU). The cellular lipid assay kit was used for detecting the concentration of triglyceride in mouse liver. The changes in the levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) mRNA expressions were detected by RT-PCR.Results GO-CoA-Tat significantly reduced the body weight gain of fatty liver mice, and the body weight of the HFD+GO-CoA-Tat group at week 8 (33.125±0.153) was significantly different from that of the HFD+Saline group (37.327±0.296) (P=0.014). There were significant differences in TG and TC (1.237±0.071, 2.403±0.151) in HFD+GO-CoA-Tat group and HFD+Saline group (1.943±0.136, 5.013±0.369) (P=0.038 and 0.029, respectively). The GLU of HFD+GO-CoA-Tat group (4.103±0.184) was significantly different than that of HFD+Saline group (6.370±0.296) (P=0.013). Compared with the HFD+Saline group (101.332±2.052), the liver triglyceride content of mice in HFD+GO-CoA-Tat group was significantly different (75.602±1.487) (P=0.027). There were significant differences in ALT and AST in the HFD+GO-CoA-Tat group (37.862±2.425, 122.932±6.907) and the HFD+Saline group (54.901±5.201, 173.321±13.971) (P=0.035 and 0.026, respectively). There were significant differences in TNF-α and IL-6 between the HFD+GO-CoA-Tat group (1.730±0.040, 1.630±0.040) and the HFD+Saline group (2.740±0.140, 2.980±0.070) (P=0.042 and 0.038, respectively).Conclusion GO-CoA-Tat has protective roles in NAFLD mice.

Key words: Nonalcoholic fatty liver disease, High fat diet, Ghrelin o-acyltransferase, Male mice, C57bl/6

张绍仁, 胡静娴, 周皓, 蒋淼, 樊晓明. GO-CoA-Tat对非酒精性脂肪性肝病小鼠的肝损伤保护作用[J]. 肝脏, 2022, 27(11): 1213-1215.

ZHANG Shao-ren, HU Jing-xian, ZHOU Hao, JIANG Miao, FAN Xiao-ming. Protective effect of GO-CoA-Tat on liver injury of mice with non-alcoholic fatty liver disease[J]. Chinese Hepatolgy, 2022, 27(11): 1213-1215.

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