HBV相关肝细胞癌患者血清miR-504、miR-200a、IL-24水平及其与肝癌的相关性

肝脏 ›› 2023, Vol. 28 ›› Issue (10): 1186-1190.

HBV相关肝细胞癌患者血清miR-504、miR-200a、IL-24水平及其与肝癌的相关性

成晓梅, 田红, 李想, 杨洁

710054 西安空军军医大学空军第九八六医院消化内科(成晓梅,李想),肾脏病科(田红),妇产科(杨洁)

收稿日期:2023-05-23 出版日期:2023-10-31 发布日期:2023-12-06
通讯作者: 杨洁,Email:yangjie451@126.com

Analysis of serum levels and correlation of miR-504, miR-200a and IL-24 in patients with HBV-related hepatocellular carcinoma

CHENG Xiao-mei¹, TIAN Hong², LI Xiang¹, YANG Jie³

1. Department of Gastroenterology,Air Force 986th Hospital,Xi′an 710054,China;
2. Department of Nephrology,Air Force 986th Hospital,Xi′an 710054,China;
3. Department of Obstetrics and Gynecology,Air Force 986th Hospital, Xi′an 710054,China

Received:2023-05-23 Online:2023-10-31 Published:2023-12-06
Contact: YANG Jie, Email:yangjie451@126.com

摘要/Abstract

摘要： 目的探究乙型肝炎病毒(HBV)相关肝细胞癌患者血清微小RNA(miR)504、miR-200a、白细胞介素(IL)24水平及与肝癌的相关性。方法选取2020年2月至2023年2月于空军第986医院接受治疗且符合纳入、排除标准的HBV相关性肝细胞癌患者60例作为观察组,选取同期慢性HBV慢性感染者60例作为对照组。对比两组患者一般资料,实时荧光定量PCR测定患者血清miR-504及miR-200a水平,通过酶联免疫吸附实验测定患者血清IL-24水平。通过相关性分析及二元logistic回归分析筛选与HBV慢性感染者肝癌发生相关的危险因素,采用受试者工作特征曲线下面积(AUC)评价各因素对患者肝癌发生的诊断价值。结果观察组患者HBV DNA高于对照组[(21.87±4.64)×10³比(13.61±3.36)×10³ IU/mL,P<0.05],余一般资料无显著差异。观察组患者血清miR-504(0.75±0.16比1.09±0.20)、miR-200a(0.79±0.24比1.01±0.28)及IL-24(187.65±19.04比213.25±20.27)水平均显著低于对照组患者。Spearman相关性分析及二元logistic回归分析表明血清miR-504、miR-200a及IL-24水平降低均是HBV慢性感染者肝癌发生的独立危险因素(P<0.05)。血清miR-504对HBV慢性感染者肝癌发生的诊断价值最高(AUC=0.898, P<0.05)。结论 HBV相关肝细胞癌患者血清miR-504、miR-200a、IL-24水平显著降低,与HBV慢性感染者肝癌发生呈负相关,有一定诊断价值。

关键词: 乙型肝炎病毒, 微小RNA, 白细胞介素24, 肝癌

Abstract: Objective To investigate the serum levels of micro-RNA (miR) 504, miR-200a and IL-24, and thier correlation in patients with hepatitis B virus (HBV)-related hepatocellular carcinoma. Methods We selected patients with HBV-related hepatocellular carcinoma who were treated in our hospital from February 2020 to February 2023 and met the inclusion and exclusion criteria as the observation group (N=60). The control group (N=60) consisted of patients with chronic HBV infection in the same period. The general data of the two groups were compared. The levels of serum miR-504 and miR-200a were measured by real-time fluorescence quantitative PCR, and the level of serum IL-24 was measured by enzyme-linked immunosorbent assay (Elisa). The risk factors associated with hepatocellular carcinoma in patients with chronic HBV infection were screened by correlation analysis and binary Logistic regression analysis. The diagnostic value of these factors was evaluated by the receiver working characteristic curve (ROC) and the area under the curve. Results The average HBV DNA load in the observation group was significantly higher than that in the control group (21.87 ±4.64 vs 13.61 ±3.36, P<0.05), but there was no significant difference in other general data. The serum levels of miR-504 (0.75±0.16 vs 1.09±0.20), miR-200a (0.79±0.24 vs 1.01±0.28) and IL-24 (187.65±19.04 vs 213.25±20.27) in the observation group were significantly lower than those in the control group. Correlation analysis and Logistic regression analysis showed that the decrease of serum miR-504, miR-200a and IL-24 levels were independent risk factors for hepatocellular carcinoma in patients with chronic HBV infection(P<0.05). ROC curve analysis showed that serum miR-504 had the highest diagnostic value for hepatocellular carcinoma in patients with chronic HBV infection (AUC=0.898, P<0.05). Conclusion The levels of miR-504, miR-200a and IL-24 in the serum of patients with HBV-related hepatocellular carcinoma showed a significant decrease. Moreover, each of these indicators exhibited a negative correlation with the occurrence of hepatocellular carcinoma in patients with chronic HBV infection. These findings suggest that the measurement of these indicators had a certain diagnostic value for HBV-related hepatocellular carcinoma.

Key words: Hepatitis B virus, micro-RNA, Interleukin-24, Hepatocellular carcinoma

成晓梅, 田红, 李想, 杨洁. HBV相关肝细胞癌患者血清miR-504、miR-200a、IL-24水平及其与肝癌的相关性[J]. 肝脏, 2023, 28(10): 1186-1190.

CHENG Xiao-mei¹, TIAN Hong², LI Xiang¹, YANG Jie³. Analysis of serum levels and correlation of miR-504, miR-200a and IL-24 in patients with HBV-related hepatocellular carcinoma[J]. Chinese Hepatolgy, 2023, 28(10): 1186-1190.

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