慢性丙型肝炎患者转录因子MafB的表达及对免疫调节功能的作用

摘要/Abstract

摘要： 目的探讨慢性丙型肝炎患者转录因子MafB的表达及对免疫调节功能的作用,同时为寻找中医辨证的分子标志物提供科学的依据。方法选取2019年8月—2022年10月期间入院诊治慢性丙型肝炎患者65例,根据中医证型分型,肝郁脾虚证型为41例(肝郁脾虚组),其他证型(肝胆湿热型、肝肾阴虚型和淤血阻络型)24例(其他证型组)。纳入同期健康人59例。比较不同组别临床特征和免疫学相关指标。结果慢性丙型肝炎组ALT水平显著高于健康对照组,差异具有统计学意义(P<0.05)。比较免疫学相关指标可知,慢性丙型肝炎组单核细胞MafB mRNA相对表达水平和单核细胞MafB蛋白相对丰度值显著高于健康对照组,血清INF-α1水平和血清INF-β水平显著低于健康对照组,IL-4水平和IL-12水平显著高于健康对照组,差异具有统计学意义(P<0.05)。基因型2a和基因型3丙型肝炎病毒感染者血清INF-α1水平显著低于基因型1b丙型肝炎病毒感染者,差异具有统计学意义(P<0.05)。其他证型组丙型肝炎病毒感染者INF-α1显著低于肝郁脾虚组丙型肝炎病毒感染者,差异具有统计学意义(P<0.05)。相关性分析得出MafB表达水平与血清INF-α1显著负相关,与血清IL-4水平正相关(P<0.05)。结论慢性丙型肝炎患者转录因子MafB表达水平升高,且可以导致慢性丙型肝炎患者发生免疫系统严重的调节紊乱,这种免疫功能的失调可能是丙型肝炎进展为为慢性病的主要原因之一。

关键词: 慢性丙型肝炎, 淋巴细胞, 转录因子

Abstract: Objective To investigate the expression of transcription factor MafB and its role in immunomodulatory function in patients with chronic hepatitis C, and to provide a scientific basis for finding molecular markers for syndrome differentiation in traditional Chinese medicine (TCM). Methods Between August 2019 and October 2022, 65 patients with chronic hepatitis C were selected. According to TCM syndrome types, 41 cases were liver depression and spleen deficiency syndrome (liver depression and spleen deficiency group) and 24 cases were other TCM types, such as liver and gallbladder damp-heat type, liver and kidney yin deficiency type and blood stasis blocking collateral type (other syndromes group). 59 healthy people in the same period were divided into healthy control group. The clinical features and immunological indexes of different groups were compared. Results The ALT level in chronic hepatitis C group was significantly higher than that in healthy control group, and the difference was statistically significant (P<0.05). Comparing the immunological indexes, we can know that the relative expression level of monocyte MafB mRNA, the relative abundance value of monocyte MafB protein, IL-4 and IL-12 levels in chronic hepatitis C group are significantly higher than those in healthy control group, and the levels of serum INF-α1 and INF-β are significantly lower than those in healthy control group, with statistical significance (P<0.05). In subgroup analysis of hepatitis C virus (HCV) genotypes, the levels of serum INF-α1 in HCV genotypes 2a and 3 were significantly lower than those in genotype 1b, and the difference was statistically significant (P<0.05). The INF-α1 of HCV infection in other syndrome groups was significantly lower than that in the group of liver depression and spleen deficiency, and the difference was statistically significant (P<0.05). Correlation analysis showed that MafB expression level was negatively correlated with serum INF-α1, It is positively correlated with serum IL-4 levels (P<0.05). Conclusion The increased expression of transcription factor MafB in patients with chronic hepatitis C and inhibition of immunomodulatory function in patients with chronic hepatitis C may be one of the important reasons for the chronic infection of HCV.

Key words: Chronic hepatitis C, Lymphocyte, Transcriptional factor

李珉, 荆巧玲, 常柏. 慢性丙型肝炎患者转录因子MafB的表达及对免疫调节功能的作用[J]. 肝脏, 2024, 29(9): 1100-1104.

LI Min, JING Qiao-ling, CHANG Bai. Expression of transcription factor MafB and its effect on immune regulation in patients with chronic hepatitis C[J]. Chinese Hepatolgy, 2024, 29(9): 1100-1104.

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