关键词: miR-4262, miR-934, 原发性肝癌, 病理特征

Abstract: Objective To investigate the relationship among miR-4262, miR-934 and the clinical pathological characteristics and prognosis of patients with primary liver cancer. Methods 50 patients with primary liver cancer who received treatment in our hospital from June 2019 to March 2021 were selected as the observation group, and 50 healthy individuals who underwent physical examinations during the same period were selected as the control group. Real time fluorescence quantitative PCR and differential analysis were used to detect the levels of miR-4262 and miR-934 in serum, liver cancer tissue, and adjacent tissues, respectively, and compare the differences between the two groups. The relationship between the expression of miR-4262 and miR-934 and clinicopathological features was analyzed. After a 3-year follow-up of primary liver cancer patients, the Kaplan Meier survival curves was plotted to analyze the relationship between miR-4262, miR-934 and survival time of primary liver cancer patients. Results The relative expression levels of miR-4262 and miR-934 in the serum of the observation group were 1.75 ± 0.13 and 2.47 ± 0.33, respectively, which were significantly higher than those of the control group (1.02 ± 0.02 and 1.13 ± 0.05, respectively), and the difference was statistically significant (P<0.05). The relative expression levels of miR-4262 and miR-934 in cancer tissues were 1.84 ± 0.21 and 2.52 ± 0.41, respectively, which were significantly higher than those in adjacent tissues of 0.97 ± 0.16 and 0.86 ± 0.32 (P<0.05). There were significant differences in the expression levels of miR-4262 and miR-934 among liver cancer patients in terms of lymphatic vessel metastasis, tumor staging, and vascular invasion (P<0.05). The three-year cumulative survival rate of patients with high expression of miR-4262 in liver cancer tissue was 33.33% (8/24), and 53.85% (14/26) of patients with significantly low expression (P<0.05). The three-year cumulative survival rate of miR-934 high expression patients was 37.04% (10/27), significantly lower than the 52.17% (12/23) of low expression patients (P<0.05. The results of multiple logistic regression analysis indicated that lymph node metastasis and vascular invasion were independent risk factors affecting the prognosis of patients. Conclusion MiR-4262 and miR-934 are closely related to lymph node metastasis and vascular invasion in patients with primary liver cancer, and have a certain prognostic value.

Key words: miR-4262, miR-934, Primary liver cancer, Pathological features