隐匿性乙型肝炎病毒感染情况调查

肝脏 ›› 2018, Vol. 23 ›› Issue (9): 775-777.

隐匿性乙型肝炎病毒感染情况调查

夏利芳, 关博文, 单耀斌, 李昆鹏, 王童, 郭桐生

100039 北京解放军第三○二医院临床检验中心(夏利芳,单耀斌,李昆鹏,王童,郭桐生);河北北方学院医学检验学院2015级5班(关博文)

收稿日期:2018-05-11 出版日期:2018-09-30 发布日期:2020-04-26
通讯作者: 郭桐生,Email:gtsheng@sina.com

Investigation of occult hepatitis B virus infection

XIA Li-fang, GUAN Bo-wen, SHAN Yao-bin, LI Kun-peng, WANG Tong, GUO Tong-sheng

302^nd Hospital of PLA, Center of Clinical Laboratory, Beijing 100039,China

Received:2018-05-11 Online:2018-09-30 Published:2020-04-26
Contact: GUO Tong-sheng, Email:gtsheng@sina.com

摘要/Abstract

摘要： 目的调查隐匿性HBV感染(OBI)的情况。方法连续收集2016年8月至2017年3月HBsAg(-)且其他HBV标志物阳性患者血清样本1570份,同时排除单独抗-HBs阳性患者。采用全自动磁珠法核酸提取及实时荧光定量PCR系统进行HBV DNA定量检测,同时采用罗氏CAP/Prep HBV检测系统复核。结果 1570份标本中共有71份HBV DNA阳性标本。1570份标本中,肝炎506份,肝硬化399份,肝占位41份,肝癌189份,其他诊断435份。HBV DNA阳性标本中,肝炎31份,肝硬化7份,肝占位3份,肝癌16份,其他14份。71份OBI标本中有7份为健康查体者标本。结论 OBI在人群中有一定的发生率,肝占位和肝癌患者OBI发生率较高,健康体检人群中仍可筛查出OBI者。

关键词: 乙型肝炎隐匿性感染, 乙型肝炎病毒表面抗原, 乙型肝炎病毒脱氧核糖核酸

Abstract: Objective To investigate occult hepatitis B virus infection (OBI).Methods From August 2016 to March 2017, 1570 serum samples were collected continuously from hepatitis B surface antigen (HBsAg) negative patients who had at least any other positive marker in hepatitis B virus (HBV) markers. Patients with only HBsAb positive were excluded. High sensitive nucleic acid detection, full-automatic magnetic beads nucleic acid extraction and fluorescence quantitative polymerase chain reaction were used for HBV DNA quantitative detection. According to the instructions, HBV DNA >20 IU/mL was defined as positive. Meanwhile, Roche CAP/Prep HBV detection system was used to check the results. Results There were 71 HBV DNA-positive specimens in 1570 specimens, of which 49 were males and 22 were females. All cases were divided into 5 groups according to the diagnosis, including 506 cases of hepatitis, 399 cases of liver cirrhosis, 41 cases of liver space occupying lesion, 188 cases of liver cancer and 436 cases of other diagnoses. Of the 71 HBV DNA positive specimens, there were 31 cases of hepatitis (6%), 7 cases of liver cirrhosis (2%), 3 cases of liver space occupying lesion (7%), 16 cases of liver cancer (8%) and 14 other cases (3%). Moreover, 7 of the 71 HBV DNA positive specimens were from health examination.Conclusion OBI can occur in the population especially develop liver occupying lesion and liver cancer, and its screening should also be done in healthy people.

Key words: Occult hepatitis B virus infection, Hepatitis B surface antigen, Hepatitis B virus DNA

夏利芳, 关博文, 单耀斌, 李昆鹏, 王童, 郭桐生. 隐匿性乙型肝炎病毒感染情况调查[J]. 肝脏, 2018, 23(9): 775-777.

XIA Li-fang, GUAN Bo-wen, SHAN Yao-bin, LI Kun-peng, WANG Tong, GUO Tong-sheng. Investigation of occult hepatitis B virus infection[J]. Chinese Hepatolgy, 2018, 23(9): 775-777.

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