细胞周期激酶亚单位1对肝细胞癌细胞增殖能力的影响

肝脏 ›› 2016, Vol. 21 ›› Issue (7): 545-548.

细胞周期激酶亚单位1对肝细胞癌细胞增殖能力的影响

金宇霆, 张江, 武昊宇, 夏强

200127 上海交通大学医学院附属仁济医院肝脏外科

收稿日期:2016-03-02 出版日期:2016-07-31 发布日期:2020-07-09
通讯作者: 夏强,Email:xiaqiang@medmail.com.cn
基金资助:
国家自然基金项目(81472243)

Effect of cyclin dependent kinase submit 1 on proliferation of hepatocellular carcinoma(HCC) BEL-7405 cells

JIN Yu-ting, ZHANG Jiang, WU Hao-yu, XIA Qiang

Department of Liver Surgery, RenJi Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China

Received:2016-03-02 Online:2016-07-31 Published:2020-07-09
Contact: XIA Qiang, Email: xiaqiang@medmail.com.cn

摘要/Abstract

摘要： 目的观察细胞周期激酶亚单位1(CKS1)在肝细胞癌及正常肝组织中的表达以及其对人肝细胞癌细胞BEL-7405的增殖能力的影响。方法应用免疫组织化学检测65对手术肝细胞癌组织及癌旁正常组织CKS1的表达水平。应用Western印迹检测肝癌细胞系与正常肝脏细胞中CKS1表达量的差异。应用小干扰RNA(siRNA)技术转染肝细胞癌BEL-7405细胞,Western印迹检测CKS1 siRNA对CKS1蛋白的干扰效果,同时应用细胞计数试剂盒(CCK-8)和平板克隆增殖实验检测CKS1对BEL-7405细胞增殖能力的影响。结果免疫组织化学染色结果显示,CKS1在正常肝组织中表达量较肝细胞癌组织中表达量低,且差异有统计学意义(P<0.05)。Western印迹检测表明CKS1在7种肝细胞癌细胞系表达量显著高于正常肝细胞株,且CKS1的表达量在肝细胞癌BEL-7405细胞株显著高于其他肝癌细胞株。CCK-8法检测CKS1的siRNA转染BEL-7405细胞与阴性对照组和空白对照组相比明显抑制细胞增殖,在48、72和96 h A值分别降低了0.376、0.566、0.972(P<0.05);平板克隆增殖形成实验表明,CKS1的siRNA转染BEL-7405细胞后肿瘤细胞形成的克隆增殖集落明显少于阴性对照组和空白组,干扰组与空白、阴性对照组比较差异有统计学意义(P<0.05)。结论 CKS1在肝细胞癌组织中表达量显著高于正常肝组织,在肝癌细胞中表达量显著高于正常肝细胞, CKS1在肝细胞癌细胞BEL-7405的增殖中扮演了重要的角色,CKS1可能成为针对肝细胞癌基因治疗的新兴靶点。

关键词: 肝细胞癌, 细胞周期激酶亚单位1, siRNA干扰, 细胞增殖

Abstract: Objective To investigate the expression of cyclin dependent kinase submit 1 (CKS1) in hepatocellular carcinoma (HCC) and its effect on the proliferation of HCC cell line, BEL-7405.Methods The expression of CKS1 in 65 pairs of HCC tissues and normal tissues was measured by immunohistochemistry (IHC). Meanwhile, the expression of CKS1 in 7 different HCC cell lines and normal liver cell line was analyzed by western blot. Furthermore, a small interfering RNA (siRNA) targeting CKS1 was constructed and transfected into human HCC cells BEL-7405. The effects of CKS1 knockdown on cell proliferation and gene expression were assessed by CCK-8 assay, tablet cloning assay and western blot analysis, respectively.Results CKS1 expression was extremely higher in HCC tissue than that in normal liver tissue (P<0.05). Proliferation of siRNA-transfected BEl-7405 cells (siRNA-transfected group) was extremely inhibited comparing to that of negative control or blank group (P<0.05).Conclusion The expression of CKS1 in HCC tissues and cell lines was significantly higher than that normal liver tissue or cell line, respectively. Additionally, CKS1 plays an important role in proliferation of BEL-7405 HCC cell lines, which might be a novel anti-tumor target for HCC.

Key words: HCC, CKS1, siRNA, Cell proliferation

金宇霆, 张江, 武昊宇, 夏强. 细胞周期激酶亚单位1对肝细胞癌细胞增殖能力的影响[J]. 肝脏, 2016, 21(7): 545-548.

JIN Yu-ting, ZHANG Jiang, WU Hao-yu, XIA Qiang. Effect of cyclin dependent kinase submit 1 on proliferation of hepatocellular carcinoma(HCC) BEL-7405 cells[J]. Chinese Hepatolgy, 2016, 21(7): 545-548.

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