氧化苦参碱改善肝硬化大鼠细胞凋亡性肠黏膜损伤的机制

肝脏 ›› 2019, Vol. 24 ›› Issue (1): 74-78.

氧化苦参碱改善肝硬化大鼠细胞凋亡性肠黏膜损伤的机制

文剑波, 陈卫国, 张潇, 江丽萍, 胡兆鹏, 周云

337000 江西萍乡市人民医院消化内科(文剑波,陈卫国,张潇),药物临床试验机构(陈杰),动物实验室(江丽萍),病理科(胡兆鹏),进贤县人民医院内四科(周云)

收稿日期:2018-03-17 出版日期:2019-01-31 发布日期:2020-04-09
通讯作者: 文剑波,E-mail:wenjbpx@126.com

Received:2018-03-17 Online:2019-01-31 Published:2020-04-09

摘要/Abstract

摘要： 目的探究氧化苦参碱(oxymatrine,OMT)在肝硬化大鼠肠黏膜损伤改善中与肠上皮细胞凋亡的关系。方法 SD大鼠30只,随机分为空白组(4只)、肝硬化造模组(26只)。空白组:常规饲养9周后,按体质量肌注5%葡萄糖水4周,消毒饲料及水自由摄入,标记为空白对照组A组;肝硬化造模组:采用改良四因素复合造模法9周后制成肝硬化成模大鼠(16只),随机选择8只予以40% CCl₄橄榄油溶液皮下注射及按体质量肌注5%葡萄糖水4周,标记为模型组B组;剩余8只予以40% CCl₄橄榄油溶液皮下注射及按体质量肌注氧化苦参碱葡萄糖水4周,标记为治疗组C组;消毒饲料及含甘草甜味素的10%乙醇溶液自由摄入。TUNEL法检测各组肠黏膜上皮细胞的凋亡率、RT-PCR法检测肠黏膜Bcl-2和Bax的mRNA表达。结果 B组回肠上皮细胞凋亡率明显高于A组 (P<0.01),C组凋亡率低于B组 (P<0.05);A组Bcl-2 mRNA呈现高表达,Bax mRNA呈现为低表达;B组Bcl-2 mRNA的表达明显低于A组(P<0.01),Bax mRNA表达明显高于A组(P<0.01);C组Bcl-2 mRNA的表达水平明显增加高于B组 (P<0.05),Bax mRNA的表达水平明显减少低于B组(P<0.01),均差异有统计学意义。结论 OMT通过上调肝硬化大鼠回肠组织中凋亡相关基因Bcl-2 mRNA的表达、下调Bax mRNA的表达抑制肠黏膜上皮细胞凋亡,从而改善肠黏膜损伤,可能是保护肠黏膜屏障功能的机制之一。

关键词: 氧化苦参碱, 细胞凋亡, Bcl-2, Bax

文剑波, 陈卫国, 张潇, 江丽萍, 胡兆鹏, 周云. 氧化苦参碱改善肝硬化大鼠细胞凋亡性肠黏膜损伤的机制[J]. 肝脏, 2019, 24(1): 74-78.

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