Vanin-1作为对乙酰氨基酚致急性肝损伤早期评价标志物的相关性分析

摘要/Abstract

摘要： 目的: 探讨泛酰巯基乙胺酶(Vanin-1)在对乙酰氨基酚(APAP)所致急性肝损伤早期的表达变化及评价中的应用价值。方法: 建立APAP诱导的急性肝损伤模型,将小鼠随机分为对照组、不同浓度APAP(100、200、300 mg/kg)处理组,对小鼠血清中Vanin-1及肝损伤标志物谷丙转氨酶(ALT)水平,及肝组织Vanin-1蛋白水平进行测定,对Vanin-1与ALT进行平行比对并分析其相关性,评价Vanin-1在APAP诱导的小鼠急性肝损伤早期评价中的作用。结果: (1)造模12 h后,与对照组相比,APAP处理组小鼠血清ALT水平显著上调(P<0.05),且其水平变化呈剂量依赖性;(2)造模12 h后,APAP处理组血清及肝脏组织中的Vanin-1表达水平显著高于对照组(P<0.05),且呈剂量依赖性;(3)在对急性肝损伤早期评价中,血清Vanin-1 mRNA与ALT具有相关性(R>0.9);(4)与ALT相比,Vanin-1在急性肝损伤中表达更具灵敏性。结论: Vanin-1有望成为APAP致急性肝损伤的早期评价标志物,但仍需进一步研究。

关键词: 急性肝损伤, 对乙酰氨基酚, 泛酰巯基乙胺酶1, 早期评估, 谷丙转氨酶

Abstract: Objective To investigate the expression and evaluation value of vanin-1 in acute liver injury induced by acetaminophen (APAP). Methods We established the acute liver injury model of mice induced by APAP. The rats were randomly divided into control group and experimental groups treated with various concentrations of APAP (100, 200, 300 mg/kg). The serum alanine aminotransferase (ALT) level, vanin-1 level in serum and in liver were detected. Parallel contrast was conducted between vanin-1 and ALT to analyze the correlation, and to evaluated the value of vanin-1 in the early evaluation of APAP-induced acute liver injury in mice. Results (1) At 12 hours (12h) after the establishment of the model, compared with the control group, serum ALT levels in the APAP treatment groups were significantly higher (P<0.05), which was dose-dependent. (2) At 12h, the expression levels of vanin-1 in serum and liver tissue in the APAP treatment groups were significantly higher than those in the control group (P<0.05), which was dose-dependent. (3) In the early evaluation of acute liver injury, vanin-1 mRNA was correlated with ALT (R>0.9). (4) Compared with ALT, vanin-1 is more sensitive in acute liver injury. Conclusion Vanin-1 is expected to be a marker for the early evaluation of acute liver injury induced by APAP, which needs further research.

Key words: Acute liver injury, Acetaminophen, Vanin-1, Early evaluation, Alanine aminotransferase

甄丽影. Vanin-1作为对乙酰氨基酚致急性肝损伤早期评价标志物的相关性分析[J]. 肝脏, 2020, 25(7): 679-681.

ZHEN Li-ying. Vanin-1 as a marker for early evaluation of acetaminophen induced acute liver injury[J]. Chinese Hepatolgy, 2020, 25(7): 679-681.

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