关键词: 酒精性肝病, 模型, 肝脏, 小肠, 病理

Abstract: Objective To study the injury characteristics of Gao-Binge alcoholic liver disease model in mice. Methods C57BL/6N male mice were randomly divided into normal group and model group. The normal group was fed with control liquid diet, the model group was fed with control liquid diet for 5 days, and then fed with alcohol liquid diet for 10 days. On the morning of the 16th day, the mice in the model group were given a high dose of alcohol (5 g/kg), while the mice in the normal group were fed with equal calorie maltodextrin solution (9 g/kg). Nine hours later, blood, liver and small intestine were collected to detect plasma ALT, AST activity and endotoxin levels. HE staining was used to observe the pathological injury of liver and small intestine. Western Blot was used to detect the levels of tight junction protein Zo-1 and Occludin in small intestine. Results In the model group, the liver index, ALT, AST and endotoxin levels were significantly increased(P<0.01), the liver pathology showed steatosis, intestinal barrier was damaged, intestinal vasodilation and hyperemia were found in the model group, and the levels of tight junction protein Zo-1 (P<0.05)and Occludin (P<0.01)were significantly decreased. Conclusion Gao-Binge alcoholic liver disease model can lead to liver injury and small intestinal pathological injury in mice, and the level of plasma endotoxin increases, which provides a good animal model basis for the theoretical study of intestinal-liver axis of alcoholic liver disease. It is suggested that the prevention and treatment of alcoholic liver disease should also reduce small intestinal injury and reduce the level of plasma endotoxin as one of the key points in the future.

Key words: Alcoholic liver disease, Model, Liver, Small intestine, Pathology