超声弹性成像评价急性兔有机磷中毒性肝损伤的实验研究

肝脏 ›› 2022, Vol. 27 ›› Issue (11): 1203-1205.

超声弹性成像评价急性兔有机磷中毒性肝损伤的实验研究

裴峰, 马之萍, 郭明阳, 陈婷婷, 何沈岩, 涂娇, 袁萍, 钱嵘

200052 上海海军第九○五医院超声诊断科(钱嵘,马之萍,郭明阳,陈婷婷,何沈岩,涂娇,袁萍 );中国人民武装警察部队上海总队医院医学影像科(裴峰)

收稿日期:2022-04-19 出版日期:2022-11-30 发布日期:2023-01-31
通讯作者: 钱嵘,Email:2651184151@qq.com

An experimental study on the evaluation of organophosphorus poisoning-induced acute toxic liver injury in rabbit with ultrasonic tissue elastography

PEI Feng², MA Zhi-ping¹, GUO Ming-yang¹, CHEN Ting-ting¹, HE Shen-yan¹, TU Jiao¹, YUAN Ping¹, QIAN Rong¹

1. Department of Ultrasound,the 905th Naval Hospital,Shanghai,200052,China;
2. Department of Medical Imaging, Shanghai General Hospital of the Chinese People’s Armed Police Force, Shanghai 201103, China

Received:2022-04-19 Online:2022-11-30 Published:2023-01-31
Contact: QIAN Rong,Email:2651184151@qq.com

摘要/Abstract

摘要： 目的探讨超声实时组织弹性成像(RTE)评价急性中毒性肝损伤的可行性。方法 50只健康大白兔,分对照组及染毒组,染毒组腹腔注射有机磷染毒,对照组腹腔注射0.9% NaCl。染毒组注射后4、6、8、10 h肝脏行常规超声、RTE检查及抽血查肝生化指标,并取各时间段兔肝组织行病理检查。对照组采用相同方法检测。结果染毒后8、10 h兔丙氨酸氨基转移酶(ALT)分别为(50.18±19.38)U/L、(76.53±21.31)U/L,总胆红素(TBil)分别为(10.61±2.79)μmol/L、(11.09±1.95)μmol/L,而白蛋白(Alb)分别为(36.75±16.11)g/L、(31.92±13.89)g/L(P<0.05),染毒后6 h、8 h、10 h肝脏弹性评分较对照组逐渐增加,染毒后10 h感兴趣区弹性评分≥3分。以病理结果为标准,RTE诊断肝细胞受损的敏度度为95.83%,特异度为85.71%,准确率为92.11%。结论超声弹性成像可以反映急性有机磷中毒兔肝组织病理变化,中毒后可见肝细胞气球样变、脂肪变,部分肝细胞变性、坏死。

关键词: 超声弹性成像, 肝细胞损伤, 有机磷中毒

Abstract: Objective To investigate the feasibility of ultrasonic real-time tissue elastography (RTE) in evaluating acute toxic liver injury.Methods Fifty healthy rabbits were divided into a control group and a poisoning group. The poisoning group was intraperitoneally injected with organophosphate and the control group was treated with normal saline. Routine ultrasonography, RTE and blood sampling were performed pre-poisoning and at 4 h, 6 h, 8 h, 10 h after organophosphorus exposure. Meanwhile, rabbit liver tissues were taken for pathological examination. The same procedures were performed in the control group.Results At 8 hours and 10 hours after organophosphorus exposure, alanine aminotransferase (ALT) and total bilirubin (TBIL) increased (P<0.05), the levels of ALT were (50.18 ± 19.38) and (76.53 ± 21.31), and the levels of TBil were (10.61 ± 2.79) and (11.09 ± 1.95), respectively. On the contrary, the levels of albumin (Alb) decreased, (36.75 ± 16.11) and (31.92 ± 13.89), respectively (P<0.05). At 6 hours, 8 hours and 10 hours after exposure, the liver elasticity score gradually increased when compared with the control group. At 10 hours after exposure, the elasticity score of the interested region ≥ 3 points. Based on the pathological diagnostic criteria, the sensitivity, specificity and accuracy of RTE in the diagnosis of hepatocyte injury were 95.83 %, 85.71% and 92.11%, respectively.Conclusion Ultrasound elastography may reflect the pathological changes of liver tissue in rabbits with acute organophosphorus poisoning, which include hepatocellular ballooning, steatosis and degeneration and necrosis.

Key words: Ultrasonic tissue elastography, Hepatocyte injury, Organophosphorus poisoning

裴峰, 马之萍, 郭明阳, 陈婷婷, 何沈岩, 涂娇, 袁萍, 钱嵘. 超声弹性成像评价急性兔有机磷中毒性肝损伤的实验研究[J]. 肝脏, 2022, 27(11): 1203-1205.

PEI Feng, MA Zhi-ping, GUO Ming-yang, CHEN Ting-ting, HE Shen-yan, TU Jiao, YUAN Ping, QIAN Rong. An experimental study on the evaluation of organophosphorus poisoning-induced acute toxic liver injury in rabbit with ultrasonic tissue elastography[J]. Chinese Hepatolgy, 2022, 27(11): 1203-1205.

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