关键词: 肝细胞癌, 微小核糖核酸-490-3p, 微小核糖核酸-503-5p, 病理特征, 预后

Abstract: Objective To investigate the expression of microRNA (miRNA)-490-3p and miR-503-5p in hepatocellular carcinoma (HCC) and their relationship with clinicopathological features and prognosis.Methods 102 HCC patients who underwent radical resection in Handan Central Hospital from March 2018 to March 2020 were selected. The expression of miR-490-3p and miR-503-5p in HCC tissues and adjacent tissues (more than 5 cm from the edge of cancer tissues) were detected by real-time fluorescence quantitative polymerase chain reaction. The expression of miR-490-3p and miR-503-5p in cancer tissues of HCC patients with different clinicopathological features was compared. The overall survival of HCC patients with different miR-490-3p and miR-503-5p expression were analyzed by Kaplan-Meier method. Log-Rank test was used to compare the differences in survival curves between different groups. Univariate and multivariate COX risk regression models were used to analyze the prognostic factors of HCC patients.Results The relative expression levels of miR-490-3p and miR-503-5p in HCC cancer tissues were (0.86±0.23) and (0.91±0.22) respectively, which were lower than those in cancer adjacent tissues (1.90±0.31) and (2.12±0.41), with a statistically significant difference (t=27.409, 26.379, P=0.000, 0.000). The expression of miR-490-3p and miR-503-5p in HCC tissues was related to the degree of tumor differentiation, the maximum diameter of tumor, and the Chinese liver cancer staging (CNLC), and the differences were statistically significant (all P<0.05). The expression of miR-490-3p and miR-503-5p was not related to gender, age, number of tumors, HBsAg and preoperative alpha-fetoprotein, and the difference was not statistically significant (all P>0.05). The 3-year overall survival rates of miR-490-3p high expression group and low expression group were 84.62% (44/52) and 48.00% (24/50) respectively. The 3-year overall survival rates of miR-503-5p high expression group and low expression group were 87.76% (43/49) and 50.94% (27/53) respectively. The 3-year cumulative survival rate of miR-490-3p low expression group and miR-503-5p low expression group was significantly lower than that of miR-490-3p high expression group and miR-503-5p high expression group respectively, and the difference was statistically significant (Log-rank χ²=16.231, 10.604, P=0.000, 0.001). CNLC stage IIIa, tumor maximum diameter ≥5 cm, and poor differentiation were independent risk factors affecting the prognosis of HCC patients, and increased expression of miR-490-3p and miR-503-5p was a protective factor.Conclusion The decrease expression of miR-490-3p and miR-503-5p in HCC patients is relate to poor clinicopathological features and is an independent risk factor affecting the prognosis of HCC patients.

Key words: Hepatocellular carcinoma, Microribonucleic acid-490-3p, Microribonucleic acid-503-5p, Pathological features, Prognosis