Notch2分子对慢性乙型肝炎患者CD8+T细胞功能的影响及意义

肝脏 ›› 2016, Vol. 21 ›› Issue (7): 572-575.

Notch2分子对慢性乙型肝炎患者CD8⁺T细胞功能的影响及意义

李彧, 连建奇, 张野, 王九萍, 魏欣, 张鸿, 曹宁家, 田颖, 白雪帆

710054 陕西西安陕西省人民医院感染性疾病科 (李彧、张鸿、曹宁家);第四军医大学唐都医院感染科(白雪帆、连建奇、张野、魏欣);第四军医大学西京医院感染科(王九萍);陕西省中医医院妇科(田颖)

收稿日期:2015-03-24 出版日期:2016-07-31 发布日期:2020-07-09
通讯作者: 白雪帆,Email:xfbai2011@163.com
基金资助:
陕西省自然科学基金资助项目(编号:2013JQ4013)

Received:2015-03-24 Online:2016-07-31 Published:2020-07-09

摘要/Abstract

摘要： 目的观察慢性乙型肝炎患者外周血CD8⁺T细胞中Notch2分子的表达情况,并分析Notch2分子对CD8⁺T细胞功能的影响。方法选择30例慢性乙型肝炎患者和15名健康对照者,应用密度梯度离心法分离外周血单个核细胞,应用免疫磁珠法分离CD8⁺T细胞,应用反转录实时定量PCR法检测CD8⁺T细胞中Notch2 mRNA的表达水平。将分离的CD8⁺T细胞与HepG2.2.15细胞混合培养,并加入抗Notch2抗体,培养48 h后应用CCK-8法检测细胞增殖,应用ELISA法检测上清中IFN-γ、IL-2及TNF-α的表达水平。结果慢性乙型肝炎患者CD8⁺T细胞中Notch2 mRNA的表达水平明显升高,较健康对照者升高超过5倍。应用抗Notch2抗体抑制Notch2分子信号通路后,混合培养细胞的增殖水平(8.88±1.56)×10⁵较同型对照组(4.47±1.13)×10⁵和空白对照组(4.39±0.95)×10⁵均明显升高,培养上清中的IFN-γ:3815±807 pg/mL和TNF-α:346.3±77.3 pg/mL的表达水平也较同型对照组IFN-γ:1316±484 pg/mL;TNF-α:139.8±40.1 pg/mL及空白对照IFN-γ:1383±604 pg/mL;TNF-α:124.0±45.5 pg/mL组显著升高,差异均具有统计学意义。结论 HBV感染可能通过提高Notch2的表达抑制CD8⁺T细胞的杀伤功能,诱导机体免疫耐受,导致感染慢性化。

关键词: 慢性乙型肝炎, Notch1, Notch2, CD8⁺T淋巴细胞

李彧, 连建奇, 张野, 王九萍, 魏欣, 张鸿, 曹宁家, 田颖, 白雪帆. Notch2分子对慢性乙型肝炎患者CD8⁺T细胞功能的影响及意义[J]. 肝脏, 2016, 21(7): 572-575.

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