祛脂三味方对非酒精性脂肪性肝病大鼠血脂和氧化应激的影响

肝脏 ›› 2016, Vol. 21 ›› Issue (7): 579-583.

祛脂三味方对非酒精性脂肪性肝病大鼠血脂和氧化应激的影响

郑册, 陶然, 张琴

200336 上海上海交通大学医学院附属同仁医院感染科

收稿日期:2016-04-05 出版日期:2016-07-31 发布日期:2020-07-09
通讯作者: 张琴,Email:zhangq1030@163.com
基金资助:
上海市中医药三年行动计划项目(ZY3-CCC-3-3035);上海市优秀适宜技术项目(2013SY072)

Received:2016-04-05 Online:2016-07-31 Published:2020-07-09

摘要/Abstract

摘要： 目的探索祛脂三味方(QZSWF)对非酒精性脂肪性肝病大鼠血脂和氧化应激的影响及机理。方法 SD雄性大鼠,通过连续5周高脂饮食建立脂肪肝模型,考察中药祛脂三味方连续给药4周后对非酒精性脂肪性肝病大鼠血脂和氧化应激的影响。结果祛脂三味方组与模型组比较低密度脂蛋白(LDL)明显降低(P<0.05),与正常组比较差异无统计学意义;祛脂三味方组与模型组比较还原型谷胱甘肽(GSH)显著升高(P<0.05),与正常组比较差异无统计学意义;各组大鼠体重变化和各组肝脏重量差异无统计学意义(P>0.05)。大体标本显示,祛脂三味方组与模型组以局灶型脂肪变为主,偶见弥漫性脂肪变;病理切片示祛脂三味方组肝脏脂肪变细胞向汇管区以放射状排列。结论祛脂三味方具有降血脂和抗氧化作用,其机理主要是通过降低LDL、升高还原型谷胱甘肽(GSH)。

关键词: 脂肪肝, 血脂, 氧化应激, 祛脂三味方

郑册, 陶然, 张琴. 祛脂三味方对非酒精性脂肪性肝病大鼠血脂和氧化应激的影响[J]. 肝脏, 2016, 21(7): 579-583.

参考文献

[1] Chalasani N, Younossi Z, Lavine JE, et al. The diagnosis and management of non-alcoholic fatty liver disease: practice Guideline by the American Association for the Study of Liver Diseases, American College of Gastroenterology, and the American Gastroenterological Association. Hepatology, 2012, 55: 2005-2023.
[2] Watanabe S, Hashimoto E, Ikejima K, et al. Evidence-based clinical practice guidelines for nonalcoholic fatty liver disease/nonalcoholic steatohepatitis. Hepatol Res, 2015, 45: 363-377.
[3] 常彬霞,邹正升,李保森,等. 2015年日本胃肠病学会非酒精性脂肪性肝病/非酒精性脂肪性肝炎的循证医学临床治疗指南. 临床肝胆病杂志, 2015, 31: 1027-1030.
[4] 朱鹏, 徐宗, 王宇明. 世界胃肠病学会全球指南:非酒精性脂肪性肝病及非酒精性脂肪性肝炎. 临床肝胆病杂志, 2014,30:842-845.
[5] 范建高, 庄辉, 黄薏. 全球首部“科普版”《脂肪肝防治指南》10大关键词(一). 肝博士, 2015,2:15-21.
[6] Day CP. From fat to inflammation. Gastroenterology, 2006, 130: 207-210.
[7] Fan JG, Farrell GC. Epidemiology of non-alcoholic fatty liver disease in China. J Hepatol, 2009, 50: 204-210.
[8] Tessari P, Coracina A, Cosma A,et al. Hepatic lipid metabolism and non-alcoholic fatty liver disease. Nutr Metab Cardiovasc Dis, 2009, 19: 291-302.
[9] Lewis JR, Mohanty SR. Nonalcoholic fatty liver disease: a review and update. Dig Dis Sci, 2010, 55: 560-578.
[10] Rolo AP, Teodoro JS, Palmeira CM. Role of oxidative stress in the pathogenesis of nonalcoholic steatohepatitis. Free Radic Biol Med, 2012, 52: 59-69.
[11] 范建高. 脂肪性肝病. 北京:人民卫生出版社, 2005.
[12] 谢萍, 周新喜, 张琴. 祛脂三味方结合温针治疗脂肪性肝病70例临床观察. 上海中医药杂志, 2010, 44: 27-29.
[13] 许佳年,张琴,谢萍,等.针药结合治疗脂肪性肝病的临床观察. 上海中医药杂志, 2011, 45: 55-57.
[14] 张毅明,张琴,许佳年,等.针药并用治疗脂肪肝疗效观察. 上海针灸杂志, 2011,30: 291-292.
[15] 曹亚丽.无黄疸型肝炎证治-秦伯未治疗肝病经验.中国社区医师, 2007, 23: 31-32.
[16] Sanyal AJ, Campbell-Sargent C, Mirshahi F, et al. Nonalcoholic steatohepatitis: association of insulin resistance and mitochondrial abnormalities. Gastroenterology, 2001, 120: 1183-1192.
[17] Nobili V, Pastore A, Gaeta LM, et al. Glutathione Metabolism and antioxidant enzymes in patients affected by nonalcoholic steatohepatitis. Clinica chimica acta, 2005, 355: 105-111.
[18] Adinolfi LE, Utili R, Andreana A, et al. Serum HCV RNA levels correlate with histological liver damage and concur with steatosis in progression of chronic hepatitis C. DigDis Sci, 2001, 46: 1677-1683.