关键词: 自身免疫性肝炎, AMA, 抗LC-1抗体, IL-17, 自身抗体

Abstract: Objective To evaluate the clinical diagnostic value of combined detection of anti-mitochondrial antibody(AMA),Anti-lc-1 antibody and interleukin-17(IL-17) in autoimmune hepatitis. Methods The study included 50 healthy individuals who underwent physical examination at our hospital from January 2023 to January 2024 as the healthy group, and 50 patients with autoimmune hepatitis diagnosed at our hospital during the same period as the AIH group, 50 patients with viral hepatitis diagnosed at our hospital during the same period as the viral group.IL-17 index, the positive detection rate of AMA and anti-LC-1 antibody, the distribution and positive rate of AMA titer, and the detection rate of AMA karyotypes were compared among the three groups, and the levels of AMA, anti-LC-1 antibody and IL-17 were observed between the viral group and the immune group. The ROC curves of AMA, anti-LC-antibody-1 and IL-17 were evaluated separately and in combination. Results The levels of IL-17, direct bilirubin (DBIL), and total bilirubin (TBIL), aspartate aminotransferase (AST),alanine aminotransferase (ALT), alkaline phosphatase (ALP), γ-glutamyltransferase (GGT) in the AIH group were higher than those in the healthy group, while the white-to-red blood cell ratio was lower than that in the healthy group, with statistically significant differences (P<0.05). There was no significant difference in the levels of DBIL,TBIL,AST,ALT, ALP, GGT, and the ratio of white blood cells to red blood cells between the AIH group and the virus group (P>0.05). The IL-17 level in the immunized group was 29.1±3.5 pg/mL, the IL-17 level in the virus group was 18.3±3.1 pg/mL, and the IL-17 level in the healthy group was 13.5±2.1 pg/mL. The immunized group was significantly higher than the healthy group and the virus group, with statistically significant differences t=12.25, 11.64, P<0.05.The positive rate of AMA, anti-LC-1 antibody and related antibody combined detection in immune group was higher than that in healthy group and viral group, and the difference was statistically significant (P<0.05). The AUC of AMA for diagnosing autoimmune hepatitis was 0.774, with a 95% CI of 0.690~0.857; The AUC of anti-LC-1 antibody diagnosis was 0.57, with a 95% CI of 0.505~0.635; The AUC of IL-17 diagnosis was 0.83, with a 95% CI of 0.737~0.924; When the three indicators were jointly detected, the AUC was 0.958, and the 95% CI was 0.917~0.998. Conclusion This study demonstrates that combined detection of AMA, anti-LC-1 antibody, and IL-17 has high accuracy in the clinical diagnosis of autoimmune hepatitis, providing reliable data support for clinical practice and facilitating early detection and effective treatment of the disease.

Key words: Autoimmune hepatitis, AMA, Anti-LC-1 antibody, IL-17, Autoantibodies